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酵母浸（出）粉
96孔磁珠病毒ＤＮＡ／ＲＮＡ共提取试
蛋白-抗血清
XL1-Blue MRF’
AdMax腺病毒pBHGloxde
逆病毒载体（Clontech
LacSwith II哺乳动物
pHY300plk
ExAssist interferenc
昆虫细胞表达系统ZYCY10P3S2T E.coliZYCY10P3S2T E.coli Minicircle producer competent cells havebeen prepared and tested by a modification of the procedure ofKay (Kay MA, He CY, Chen ZY. 2010, Nature Biotechnology).These cells are suitable for the cloning of minicircle-basedplasmids. ZYCY10P3S2T E.coli minicircle producer cells arederived from a BW27783 bacterial strain that stably expresses aset of inducible minicricle-assembly enzymes, ФC31 integrase andI-SceI homing endonuclease. This bacterial strain producespurified minicircles in a time frame and quantity similar to those ofroutine plasmid DNA preparation, making it feasible to useminincircles in place of plasmids in mammalian transgeneexpression studies.Note: Other competent E. coli strains cannot be substitutedfor minicircle production as they do not have the appropriategenome modifications to produce minicircles. |
