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PE-Cy5.5标记的兔抗小鼠IgG
心电工作站
SignalSilence(R) APC3 siRNA I
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AdMax腺病毒pBHGloxde
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pHY300plk
ExAssist interferenc
昆虫细胞表达系统ZYCY10P3S2T E.coliZYCY10P3S2T E.coli Minicircle producer competent cells havebeen prepared and tested by a modification of the procedure ofKay (Kay MA, He CY, Chen ZY. 2010, Nature Biotechnology).These cells are suitable for the cloning of minicircle-basedplasmids. ZYCY10P3S2T E.coli minicircle producer cells arederived from a BW27783 bacterial strain that stably expresses aset of inducible minicricle-assembly enzymes, ФC31 integrase andI-SceI homing endonuclease. This bacterial strain producespurified minicircles in a time frame and quantity similar to those ofroutine plasmid DNA preparation, making it feasible to useminincircles in place of plasmids in mammalian transgeneexpression studies.Note: Other competent E. coli strains cannot be substitutedfor minicircle production as they do not have the appropriategenome modifications to produce minicircles. |
