产品详情
The ExAssist helper phage with XLOLR strain is designed to efficientlyexcise the pBK-CMV phagemid vector from the ZAP Express vector, whileeliminating problems associated with helper phage co-infection. TheExAssist helper phage contains an amber mutation that prevents replicationof the helper phage genome in a nonsuppressing E. coli strain such asXLOLR cells. This allows only the excised phagemid to replicate in thehost, removing the possibility of co-infection from the ExAssist helperphage. Since the ExAssist helper phage cannot replicate in theXLOLR strain, single-stranded rescue cannot be performed in this strainusing this helper phage.Mass excision can be used to generate subtraction libraries and subtractedDNA probes. Converting the library to the phagemid form also allowsscreening of the phagemid library in eukaryotic cells by transformation ofeukaryotic cells with supercoiled plasmid DNA
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