pRetroX-Tight-Pur逆病毒载体；四环素调控载体_病毒克隆载体_载体及构建_生化试剂库_商城

pRetroX-Tight-Pur逆病毒载体；四环素调控载体

产品价格: ￥17920.00

最小起订量：暂无可售数量：暂无

发货时限：: 暂无

所在地区：: 中国北京

有效期至：: 长期有效

最后更新：: 2021-07-26 18:47:04

浏览次数：: 315

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pLVX-AcGFP1-C1慢病毒系统载体 面议

载体基本信息

出品公司:	Clontech
载体名称:	pRetroX-Tight-Pur
质粒类型:	逆病毒载体；四环素调控载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	Tight
载体大小:	6568 bp
5 测序引物及序列:	--
3 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素（Puromycin）
克隆菌株:	DH5α
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	逆病毒载体pRetroX-Tight-Pur是四环素调控系统载体
产品目录号:	632104
稳定性:	稳表达
组成型/诱导型:	诱导型
病毒/非病毒:	逆转录病毒

载体质粒图谱和多克隆位点信息

pRetroX-Tight-Pur载体图谱 pRetroX-Tight-Pur 多克隆位点 pRetroX-Tight-Pur 载体特征

载体简介

pRetroX-Tight-Pur载体描述pRetroX-Tight-Pur is an inducible, self-inactivating, retroviral expression vector designed to express a gene of interest under the control of PTight, a modified Tet-responsive promoter. PTight consists of seven direct repeats of a 36 bp regulatory sequence that contains the 19 bp tet operator sequence (tetO), and a modified minimal CMV promoter (1). This vector is supplied with our Retro-XTM Tet-On Advanced and Tet-Off Advanced Inducible Expression Systems (Cat. Nos. 632104 and 632105). These systems provide ready access to the inducible gene expression strategy of Gossen & Bujard, and incorporate major improvements described by Urlinger, et al. (2-6).pRetroX-Tight-Pur is optimized to eliminate promoter interference through LTR self-inactivation. The hybrid 5’ LTR consists of the cytomegalovirus (CMV) type I enhancer and the mouse sarcoma virus (MSV) promoter. This promoter drives high levels of viral genome transcription in HEK 293-based packaging cell lines due, in part, to the presence of adenoviral E1A (7-8) in these cells. The self-inactivating feature of the vector is provided by a deletion in the 3’ LTR enhancer region (U3). During reverse transcription of the retroviral RNA, a copy of the inactivated 3’ LTR U3 region replaces the corresponding region of the 5’ LTR, resulting in the inactivation of the 5’ LTR CMV enhancer sequence. This mechanism can reduce the phenomenon known as promoter interference (9-10) and allow more efficient expression. In this way, pRetroX-Tight-Pur supports high viral titers, yet eliminates potential downstream interference between the inducible PTight promoter and its adjacent viral LTR.pRetroX-Tight-Pur contains all of the necessary viral RNA processing elements; these include the 5’ and 3’ LTRs, the packaging signal (Ψ+), and the tRNA primer binding site. For safety reasons, however, the vector lacks the structural genes (gag, pol, and env) necessary for retroviral particle formation and replication. pRetroX-Tight-Pur contains a puromycin resistance gene (Purr) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transfectants. In addition, the vector contains a ColE1 origin of replication and an E. coli Ampr gene for propagation and selection in bacteria.pRetroX-Tight-Pur can be used as either a plasmid or retroviral expression vector. When used as a retroviral expression vector, it must be transfected into a packaging cell line, such as GP2-293; we recommend the Retro-X Universal Packaging System (Cat. No. 631530). Packaging cell lines allow you to produce infectious, replication-incompetent retroviral particles. These retroviral particles can infect a wide range of target cells and transmit your gene of interest, but they cannot replicate within these cells due to the absence of viral structural genes. The separate introduction and integration of the structural genes into the packaging cell line minimizes the chance of producing replication-competent virus due to recombination events during cell proliferation.Propagation in E. coli Suitable host strains: DH5α, DH10B and other general purpose strains. Selectable marker: plasmid confers resistance to ampicillin (100 μg/ml) in E. coli hosts. E. coli replication origin: ColE1 Copy number: highNotes:The vector sequence was compiled from information in the sequence databases, published literature, and other sources, together with partial sequences obtained by Clontech. This vector has not been completely sequenced.The viral supernatants produced by this retroviral vector could contain potentially hazardous recombinant virus. Due caution must be exercised in the production and handling of recombinant retrovirus. Appropriate NIH, regional, and institutional guidelines apply.

载体序列

LOCUS       pRetroX-Tight-Pur6567 bp DNASYNDEFINITION  pRetroX-Tight-PurACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..6567                     /organism="pRetroX-Tight-Pur"                     /mol_type="other DNA"     promoter        2..506                     /label="CMV_immearly_promoter"     misc_feature    6..688                     /label="5_LTR"     misc_feature    9..296                     /label="CAG_enhancer"     misc_feature    463..483                     /label="CMV_fwd_primer"     misc_feature    758..1567                     /label="psi_plus_pack"     misc_feature    1154..1561                     /label="gag"     misc_feature    1497..1519                     /label="MSCV_primer"     misc_feature    1497..1519                     /label="pLXSN_5_primer"     misc_feature    1535..1551                     /label="pBABE_5_primer"     CDS             1669..2196                     /label="ORF frame 1"                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL                     VRKFPPAPQLASCRT*"     misc_feature    1888..1907                     /label="pCEP_fwd_primer"                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL                     VRKFPPAPQLASCRT*"     misc_feature    1890..1914                     /label="LNCX_primer"                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL                     VRKFPPAPQLASCRT*"     misc_feature    complement(2008..2031)                     /label="MSCV_rev_primer"                     /translation="MSSLLPISDRERMSSLLPISDRERMSSLLPISDRERMSSLSLSV                     IENVCRVYSLSVIENVCRGRRVRWEAYISRARLVNRQIAWRRIRGRAGSRSRTREFYR                     VGEALFPRQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPL                     VRKFPPAPQLASCRT*"     CDS             2253..3092                     /label="ORF frame 3"                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK                     PGA*"     misc_feature    2405..2424                     /label="mPGK_F_primer"                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLTMTEYKPTVRLATRDDVPRAVRT                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK                     PGA*"     CDS             complement(2418..3143)                     /label="ORF frame 2"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     gene            2493..3092                     /label="puro (variant)"                     /gene="puro (variant)"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     misc_feature    complement(3801..3823)                     /label="pGEX_3_primer"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     misc_feature    complement(3959..3979)                     /label="pBABE_3_primer"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     misc_feature    complement(3965..4180)                     /label="SV40_enhancer"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     promoter        3977..4245                     /label="SV40_promoter"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     rep_origin      4144..4221                     /label="SV40_origin"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     misc_feature    4206..4225                     /label="SV40pro_F_primer"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     gene            complement(5345..6205)                     /label="Ampicillin"                     /gene="Ampicillin"                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG                     KLGLQVERPGDEEEENSAADVRF*"     CDS             complement(5345..6205)                     /label="ORF frame 3"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(6247..6275)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        6497..506                     /label="CMV_immearly_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    6561..688                     /label="5_LTR"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc   61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac  121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata  181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc  241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta  301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac  361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc  421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc  481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc  541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc  601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat  661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc  721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc  781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta  841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc  901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc  961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag 1621 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta 1681 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt 1741 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg 1801 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg 1861 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 1921 agaaggatcc gcggccgcgc cggctctaga tcgcgaacgc gtgaattcta ccgggtaggg 1981 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 2041 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 2101 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 2161 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 2221 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 2281 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 2341 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 2401 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 2461 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc 2521 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 2581 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 2641 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 2701 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 2761 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 2821 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 2881 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 2941 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 3001 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 3061 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga 3121 ccgaaaggag cgcacgaccc catgcatcgg cgtctcgaga tatcagtggt ccaggctcta 3181 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa 3241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 3301 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag 3361 aagttcagat caaggtcagg aacagatgga acagggtcga ccctagagaa ccatcagatg 3421 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag 3481 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa 3541 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca 3601 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct 3661 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg 3721 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt 3781 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 3841 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 3901 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtagat ccggctgtgg 3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 4201 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat 4321 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa 4381 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 4441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 4501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 4561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 4621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 4681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 4741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 4801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 4861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 4921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 4981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 5521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 5581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 5641 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 5701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 5761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 5821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 5881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 5941 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 6001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 6241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 6301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 6361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt 6421 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa 6481 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt 6541 cattagttca tagcccatat atggagt//

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