Recombinant Human FGF-basic

目录号 规格 价格 说明书下载 C046A 2μg 200元  C046B 10μg 700元 C046C 50ug 1800元   FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Human and mouse mature FGF basic shares 96% sequence identity. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration.DescriptionRecombinant Human FGF-basic produced in E. coli is a non-glycosylated polypeptide chain containing 157amino acids and having a molecular mass of 17kDa.Quality ControlBiological activity: The ED50 as determined by the dose-dependant proliferation of BALB/3T3 cells was foundto be less than 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 106 IU/mg.Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was foundto be Gly-Thr-Met-Ala-Ala.Endotoxin: Less than 0.1ng/μg (1.0EU/μg) of FGF-basic as determined by LAL test.FormulationHuman FGF-basic was lyophilized from 1mg/ml solution after extensive dialysis against 20 mM phosphatebuffer, pH 7.2, 50 mM Na2SO4, 0.2 mM DTT and 0.2 mM EDTA.ReconstitutionIt is recommended to reconstitute the lyophilized rhFGF-basic in sterile PBS not less than 100μg/ml, which can then be further diluted to other solutions. Storage Lyophilized rhFGF-basic although stable at room temperature for 3 weeks, should be stored desiccated below -18°C, which is stable for up to twelve months from date of receipt. Upon reconstitution rhFGF-basic should be stored at 4°C between 2-7 days and aliquots of reconstituted samples with 0.1% HSA or BSA are stable up to 3 months below -18°C for future use.Please avoid freeze-thaw cycles.