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AKT total + Phospho S473 In-Cell ELISA试剂盒(IR)

产品价格: ¥1.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
中国广东深圳市
有效期至:
长期有效
最后更新:
2020-11-12 02:00:01
浏览次数:
531

产品详情

品牌名称:

 Akt is a serine, threonine protein kinase critical in cellular metabolism, glucose uptake, protein synthesis, cell proliferation, growth, apoptosis, survival, angiogenesis, migration and invasion. It acts downstream of the phosphatidylinositol 3 kinase (PI3K) and it mediates the effects of several growth factors such as platelet-derived growth factor, epidermal growth factor and insulin growth factor. It is activated by phosphorylation on Ser-473, Thr-308 and Tyr-474 and when active it phosphorylates transcription factors (FOXO1), kinases (GSK-3, Raf-1, ASK, Chk1) and other signaling proteins (Bad, MDM2). There are three Akt isoforms (Akt1, Akt2 and Akt3) which share 80% sequence identity also known as PKBa, PKBß and PKB?. Akt has been shown to have a role in metabolism, apoptosis and proliferation and therefore it has been proposed to be the candidate “Warburg Kinase”.

 

  • 检测方法IR
  • 测试
    1 x 96 well plate
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型Cell-based (qualitative)
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Human
  • 组件 1 x 96 tests
    1000X IRDye® Labeled Secondary Antibody Cocktail 1 x 24µl
    100X Mouse Anti Akt-1 Primary Antibody 1 x 120µl
    100X Rabbit Anti-Akt1 pS473 Primary Antibody 1 x 120µl
    100X Triton X-100 1 x 1.25ml
    10X Blocking Buffer 1 x 15ml
    10X Phosphate Buffered Saline 1 x 100ml
    1X Janus Green Stain 1 x 11ml
    400X Tween-20 1 x 4ml

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