Anti-14-3-3 theta抗体

• 特异性ab183075 detects endogenous level of total 14-3-3 theta protein.
• 经测试应用WB, IHC-Pmore details
• 种属反应性
  与反应: Mouse, Human 预测可用于: Rat, Cow, Orangutan
• 免疫原

  Synthetic peptide within Human 14-3-3 theta aa 140-154. The exact sequence is proprietary. Genbank: NP_006817 Sequence:

  QTIDNSQGAYQEAFD

  Database link: P27348

   

  Run BLAST with Run BLAST with 
• 阳性对照
  • Mouse brain, Human lymphoma and Human colon cancer tissue lysates; Human A549 and mouse NIH 3T3 cell lysates; Human tonsil tissue

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存���溶液pH: 7.30 Preservative: 0.05% Sodium azide Constituents: 50% Glycerol, 49% PBS PBS is without Mg²⁺ and Ca²⁺
• 浓度100 µl 浓度为 0.6 mg/ml 
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Signal Transduction
   
  • Adapters
   
  • Cytoplasmic

  • Stem Cells
   
  • Signaling Pathways
   
  • Wnt
   
  • 14-3-3

应用

靶标

• 细胞定位Cytoplasmic
• 数据库链接
  • Entrez Gene: 768311 Cow
  • Entrez Gene: 10971 Human
  • Entrez Gene: 22630 Mouse
  • Entrez Gene: 100171925 Orangutan
  • Entrez Gene: 25577 Rat
  • Omim: 609009 Human
  • SwissProt: Q3SZI4 Cow
  • SwissProt: P27348 Human

  see all
• 别名
  • 14-3-3 antibody
  • 14-3-3 protein T-cell antibody
  • 14-3-3 protein tau antibody

  see all

Anti-14-3-3 theta antibody 图像

• 

  Western blot - Anti-14-3-3 theta antibody (ab183075)
  All lanes : Anti-14-3-3 theta antibody (ab183075) at 1/300 dilution Lane 1 : mouse brain tissue lysate Lane 2 : Human A549 cell lysate Lane 3 : Human lymphoma tissue lysate Lane 4 : mouse NIH 3T3 cell lysate Lane 5 : Human colon cancer tissue lysate Lysates/proteins at 40 µg per lane. Secondary Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution developed using the ECL technique Predicted band size : 28 kDa Exposure time : 5 seconds
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 theta antibody (ab183075)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling 14-3-3 theta with ab183075 at a 1/20 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification: ×200)