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11，12 DHET ELISA试
- 检测方法Colorimetric
- 精确度
批次内 样品 n Mean SD CV% overall < 10% - 样品类型 Cell culture supernatant, Serum, Plasma
- 检测类型Sandwich (quantitative)
- 灵敏度 < 0.12 ng/ml
- 范围 30 ng/ml - 0.123 ng/ml
- 复检
特定样本复检结果 样品类型 平均% 范围 Serum 104.6 96% - 112% Plasma 101.4 94% - 109% Cell culture media 99.78 90% - 109% - 实验步骤Multiple steps standard assay
- 种属反应性 与反应: Mouse
- 产品概述
Abcams ACE (CD143) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse ACE (CD143) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse ACE coated on a 96-well plate. Standards and samples are pipetted into the wells and ACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse ACE antibody is added. After washing away unbound biotinylated antibody,
HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 经测试应用Sandwich ELISAmore details
- 平台Microplate
性能
- 存放说明Store at -20°C. Please refer to protocols.
-
组件 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl ACE Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-mouse ACE 2 vials Recombinant Mouse ACE Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - 研究领域
- Cardiovascular
- Blood
- Serum Proteins
- Cardiovascular
- Blood
- Blood Pressure regulation
- Cardiovascular
- Vasculature
- Vasoconstriction
- Other
- Cardiovascular
- Vasculature
- Vasodilation
- Other
- Developmental Biology
- Reproduction
- Fertilisation
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of lipids and lipoproteins
- Cancer
- Cancer Metabolism
- Cellular metabolic process
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- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Types of disease
- Cancer
- 相关性Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. Expression is thought to be subject to hormonal regulation by androgens.
- 细胞定位Cell Membrane and Secreted; Angiotensin-converting enzyme, soluble form: Secreted By similarity. Cell membrane; Single-pass type I membrane protein.
- 别名
- Angiotensin converting enzyme
- CD143
- DCP
see all - 数据库链接
- Entrez Gene: 11421 Mouse
- SwissProt: P09470 Mouse
