货号：	APZ-011
抗体名：	Anti-Shank1
抗体英文名：	Anti-Shank1
靶点：	见官方网站
浓度：	见官方网站
应用范围：	IH, WB
宿主：	Rabbit
供应商：	上海信裕生物科技有限公司
数量：	大量
级别：	高
目录编号：	APZ-011
抗原来源：	Rabbit
保质期：	6个月
适应物种：	见官方网站
标记物：	见官方网站
克隆性：	否
保存条件：	-20°C
形态：	液体或冻干粉
亚型：	见官方网站
免疫原：	见官方网站
规格：	25 µl, 50 µl, 0.2 ml

Anti-Shank1

SH3 And Multiple Ankyrin Repeat Domains 1, SPANK-1, Somatostatin Receptor-Interacting Protein
Cat #: APZ-011
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IP
Reactivity: M, R
May also work in: H

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Shank1. Anti-Shank1 antibody (#APZ-011) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Shank1 from human, rat, and mouse samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
1,2. Anti-Shank1 antibody (#APZ-011), (1:400).
3,4. Anti- Shank1 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Shank1 in rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Shank1 antibody (#APZ-011), (1:300), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Shank 1 staining (green) is detected in interneurons (vertical arrows) and in the pyramidal layer (P, horizontal arrows). Nuclei are stained with DAPI (blue).
Immunogen
Peptide (C)RSQEGRQESRSDKAK, corresponding to amino acid residues 614- 628 of rat Shank1 (Accession Q9WV48). Intracellular, between the SH3 and PDZ domains.

HomologyMouse - identical; human - 13/15 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
SpecificityThis antibody will recognize all known rat isoforms of Shank1 with the exception of isoform 2 (see Q9WV48-2).
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Shank family proteins (shank1, shank2 and shank3) are neuronal scaffold proteins that organize a cytoskeleton-associated signaling complex at the postsynaptic density (PSD) of nearly all excitatory glutamatergic synapses in the mammalian brain ¹.

Shank family, including Shank1, has five conserved protein domains: 1. ankyrin repeat domain (ANK), 2. Src homology 3 (SH3) domain, 3. PSD-95/Discs large/ZO-1 (PDZ) domain, 4. proline-rich region containing homer and cortactin-binding sites (Pro), 5. sterile α motif (SAM) domain². All these domains can act as sites for specific protein-protein interactions, and the presence of these multiple domains within a single polypeptide is consistent with a function as a scaffolding protein. Shanks interact directly or indirectly with all major types of glutamate receptors: NMDA receptors, AMPA receptors, and mGluRs via different domains. Shank proteins also interact with several actin regulatory proteins and L-type Ca²⁺ (Ca_V1.3) channels in medium spiny neurons of the striatum³.

Shank1 mRNA, being synthesized in dendritic spine, can locally control the spine morphology. The Shank1 gene is located on chromosome 19q13.33 and contains 23 exons and two alternative promoters leading to two distinct isoforms⁴. Shank1 is highly expressed in the cortex and hippocampus⁵.

Recent studies indicate that Shank1 is a causative gene for idiopathic autism spectrum disorders (ASD)¹. In addition it was found that Shank1 has a role in working memory deficits in schizophrenia⁶. Shank1 knock-out mice display increased anxiety and decreased vocal communication⁷.

References

1. Jiang, Y. H. and Ehlers, M.D. (2013) Neuron 78, 8.
2. Sheng, M. and Kim, E. (2000) J. Cell Sci. 113, 1851.
3. Stella, S.L. Jr et al.  (2012) PLoS One 7, e43463.
4. Guilmatre, A. et al.  (2014) Dev. Neurobiol. 74, 113.
5. Lim, S. et al. (1999) J. Biol. Chem.  274, 29510.
6. Lennertz, L. et al. (2012) Eur. Arch. Psychiatry Clin. Neurosci. 262, 117.
7. Hung, A.Y. et al. (2008) J. Neurosci.  28, 1697.