产品特点:
• 200bp 以上的核酸回收率高达 90%以上,核酸长度最小要求 100bp
• 有效去除 dNTP、引物、引物二聚体、盐离子和其它杂质
• 纯化后的产物在 4℃温度下保持 7 天不被降解
• 双链和单链 DNA 都能得到纯化
• 长达 18 个月有效期
• 手工操作和自动化工作站操作皆可
该试剂盒应用于:
唾液 DNA 提取、血液 DNA 提取、游离 DNAPCR 体系纯化、酶切和连接 反应体系纯化
产物适合于下游应用:
PCR、Genotyping(SNP detection)、 sequencing(Sanger and Next Generation Sequencing)、 Cloning、Primer Walking
纯化产物得率高 Utop Bead Kit 可以结合大于 100bp 的核酸片段,对于片段长度为 10KB 的长片段,也能获得 比传统过柱法高得多的得率。
Utop Bead Binding Separation Ethanol wash Elution buffer Transfer
操作步骤
1. 加入 DNA 磁珠试剂
2. 磁珠与核酸结合
3. 结合有核酸的磁珠与杂质分离
4. 乙醇洗涤
5. 加入洗脱液
6. 转移洗脱产物
1. 加入 DNA 磁珠试剂 Gently shake the Utop Bead bottle to resuspend any magnetic particles that may have settled. Add Utop Bead according to the PCR reaction volume chart below:
2. 磁珠与核酸结合 Mix reagent and PCR reaction thoroughly by pipette mixing 10 times. Let the mixed samples incubate for 5 minutes at room temperature for maximum recovery
3. 结合有核酸的磁珠与杂质分离 Place the reaction plate onto an 96 Super Magnet Plate for 2 minutes to separate beads from the solution.
4. 乙醇洗涤 Dispense 200 μ L of 70% ethanol to each well of the reaction plate and incubate for 30 seconds at room temperature. Aspirate out the ethanol and discard. Repeat for a total of two washes.
5. 加入洗脱液 Off the magnet plate, add 40 μL of elution buffer (Reagent grade water, TRIS Acetate pH 8.0, or TE) to each well of the reaction plate and pipette mix 10 times.
6. 转移洗脱产物 Place the reaction plate onto an 96 Super Magnet Plate for 1 minute to separate beads from the solution.Transfer the eluant to a new plate.
1. 根据样品数量加入适量的磁珠
2. 加入磁珠后用移液器混合 10 次,静置 5 分钟
3. 磁力吸 2 分钟,倒掉溶液,分离出吸附 DNA 的磁珠
4. 加入 200μ L 70%乙醇洗涤 30 秒洗涤 2 次
5. 用 40μ L TE 溶液洗脱,用移液器混合 10 次
6. 磁力吸 1 分钟,分离磁珠,转移提取的 DNA 到新的容器
订货信息
Utop Bead Kit – Superior Ampure XP Alternative | 10ml | B86990 | ¥1200 |
Utop Bead Kit – Superior Ampure XP Alternative | 60ml | B86991 | ¥6300 |
Utop Bead Kit – Superior Ampure XP Alternative | 450ml | B86992 | ¥36000 |
Utop Bead Kit – Superior Ampure XP Alternative | 1000ml | B86993 | ¥63000 |