Origen CP-70 CryoPur 100% DMSO

USA: For Research Use only.Disclaimer of WarrantiesOriGen Biomedical warrants that reasonable care has been used in the manufacture of this device andthat it was free from defects in workmanship or materials at the time of shipment from OriGen.OriGens sole obligation shall therefore be to repair or replace any device which it determines wasdefective at the time of shipment. Because no product is completely effective under all circumstances,and because the actual use and handling of this device is beyond our control, OriGen cannot warrantfor a good effect or against a bad effect in the application and use of this device. The buyer thereforeassumes all liability arising from any cause for damages resulting from use, misuse, use other than asintended, or resterilization of this product. OriGen therefore gives no warranty of merchantability orfitness for a particular purpose. OriGen shall not be liable for incidental or consequential loss, damageor expense resulting from the use or application of this product. This warranty is in lieu of all otherwarranties, whether implied, express, oral or written, and no individual has the authority to vary theterms of this warranty.Manufactured by: European Representative:OriGen Biomedical, Inc. OriGen Biomedical Europe7000 Burleson Road, Building D Landskronavägen 25AAustin, TX, USA 78744 25232 HelsingborgLC-45/R04 03-2012, © OriGen Biomedical, inc. All Rights Reserved1. Day J & McLelann M; Cryopreservation and Freeze drying protocols. Cryopreservationof Animal and Human Cell Lines, Humana Press, NJ, 1995, p179+2. Stiff, P.; Murgo, A.; Zaroulis, C.; Derisi, M.; Clarkson, B. Cryobiology, 20 (1983)pp.17-24.3. Hovatta, O.; Silya, R.; Krausz, T.; Abir, R.; Margara, R.; Trew, G.; Lass, A. Winston, M.Human Reproduction, 11, no.6 (1996) pp.1268-1272.4. Improved Viability of Previously Frozen Platelets, Blood, 1972 40: 509-5135. Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic UseWatt, SM, et al., Methods in Molecular Biology, 368, Jun 2007, p 237-2596. Effect of DMSO exposure without cryopreservation on hematopoietic progenitor cells.Bone Marrow Transplant. 1993 May;11(5):389-93.7. Junior AM, et al; Neurotoxicity associated with DMSO-preserved hematopoieticprogenitor cell infusion. Bone Marrow Transplantation (2008) 41, 95–96

8. American Cancer Society website

OriGen CryoPur Cryopreservation SolutionsIntended Use: OriGen CryoPur solutions are intended for use as acryopreservative media with synthesized cell cultures.Single use only: Multiple draws may be made from one vial using the VSVadapter, but do not reuse DMSO which has been exposed to cells or tissue.Sterile:. Sterilized by aseptic filtration. Do not use if not sealed.Storage:DMSO: Store in a dark well ventilated area, freezes (solidifies)at18°C. Recommended storage at 20-30°C.DMSO/Dextran: Store refrigerated: +2 to +8°CProduct Description: Dimethyl Sulfoxide (C2H6OS - DMSO) is aclear essentially odorless, hygroscopic, water-miscible liquid.Product Configuration: DMSO and DMSO/Dextran products areavailable in a variety of sizes and packages. Please see the websitefor a current listing of available configurations:

Cryoprotectant Mechanism: DMSO freely permeates the cell andmixes with water inside the cell, depressing the freezing point of thewater contained inside the cell. As the temperature decreases duringthe freezing cycle, expansion of ice crystals is inhibited, and the cellis protected from bursting. DMSO has been used to preserve avariety of cell types, including human bone marrow2, ovarian tissue3,pancreatic and platelet cells4, and hematopoietic stem cells5.Caution, Toxic: Because heat is generated when DMSO is mixedwith water, it should not be added directly to the cells.Caution, Aggressive: DMSO is a highly polar organic solvent, and isaggressive against many commonly used plastics (ABS, PVC, PC,etc). Degradation of your bag and tubing may occur, and unwantedbyproducts may be released into the sample. OriGen can supplyfreezing bags and transfer sets that are resistant to 100% DMSO.

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