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HIS*BIND PURIFICATION KIT His融合标
耐碱Protein A琼脂糖凝胶4FF预装柱
B-PER 6xHis融合蛋白纯化试剂盒
[VIP第1年] 指数:2
通过杨浦区市场监管局认证 
Beta-Actin polyclona
HRP-Goat Anti-Mouse 
HRP-Goat Anti-Rabbit
HRP-Goat Anti-Human 
Western及IP细胞裂解
RIPA裂解液(弱)
RIPA裂解液(强)
蛋白酶抑制剂混合物，| 货号： | EZIP02-10 |
| 供应商： | 上海万生昊天生物技术有限公司 |
| 规格： | 10ml |
The HA tag is derived from the human influenza hemagglutinin corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. This tag facilitates the detection, isolation, and purification of the proteins.
Isotype Mouse IgG1
Form Slurry
Source Purified antibody was conjugated to gel on optimal condition.
Concentration 50% (v/v) in PBS pH7.4, 0.02% Sodium Azide, 55% glycerol.
Storage -20℃
Applications IP, protein purification
Specificity All species
Procedure
Isolation of HA fusion proteins with Anti-HA tag antibody Affinity Gel.
1. Thoroughly suspend the Anti-HA Affinity Gel in the vial, in order to make a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.
2. Add 0.6ml TBS. Thoroughly suspend the Anti-HA Affinity Gel. Centrifuge the resin at 10000 rpm for 30 seconds. Remove the supernatant carefully. Be sure that most of the wash buffer is removed and no resin is discarded. This step should be repeated for 3-4 times.
3. Add 500μl of E .coli periplasmic extracts to the washed resin.
4. Agitate all samples gently for 2 hours at 4℃.
5. Centrifuge the resin for 30 seconds at 10000 rpm. Remove the supernatants to a fresh tube.
6. Wash the resin with 0.5ml TBS until the OD280 of the supernatant liquid＜0.05.
7. Add 10μl of 2×sample buffer to each sample (the resin and the supernatant). Boil the samples for 5 minutes.
8. Load 4μl of each sample on SDS-PAGE.
FOR RESEARCH USE ONLY.
Related Products
| Cat.# | Product | Package |
| EZIP01-1 | Anti-Flag Affinity Gel | 1ml |
| EZIP03-1 | Anti-Myc Affinity Gel | 1ml |
| EZIP04-1 | Anti-V5 Affinity Gel | 1ml |
| EZIP05-1 | Anti-His Affinity Gel | 1ml |
| EZIP06-1 | Anti-GST Affinity Gel | 1ml |
温馨提示：不可用于临床治疗。
