分子量:40.4 kDa纯度:>95% as determined by SDS-PAGE and more than 90% measured by SEC-HPLC.内毒素:Less than 1.0 EU per μg of the Human CD47, Fc Tag (HPLC-verified) by the LAL method.Buffer:50 mM tris, 100 mM glycine, pH7.5生物活性:Measured by its binding ability in a functional ELISA. Immobilized Human CD47, Fc Tag (HPLC-verified) (Catalog # CD7-H5256) at 2 μg/mL can bind Human SIRP alpha, His Tag (Catalog # SIA-H5225) with a linear range of 16-250 ng/mL.产品特性:Human CD47, Fc Tag (HPLC-verified) is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40.4 kDa. The predicted N-terminus is Gln 19. The reducing (R) protein migrates as 50-65 kDa in SDS-PAGE due to glycosylation.产品背景:Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
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