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Anti-MANEA抗体

产品价格: ¥1380.00

最小起订量:暂无 可售数量:暂无

发货时限:
暂无
所在地区:
全国
有效期至:
长期有效
最后更新:
1970-01-01 08:00:00
浏览次数:
21

产品详情

品牌名称:
Ybscience

 Anti-MANEA抗体

产品编号 YB-18652R
英文名称 MANEA
中文名称 甘露糖苷酶2抗体
别    名 2-mannosidase; 4932703L02; Alpha 1,2 endomannosidase; DKFZp686D20120; Endo alpha D mannosidase; ENDO; Endo-alpha mannosidase; Endomannosidase; FLJ12838; Glycoprotein endo alpha 1,2 mannosidase; Glycoprotein endo-alpha-1; Glycoprotein endo-alpha-1,2-mannosidase; hEndo; Mandaselin; MANEA; MANEA_HUMAN; Mannosidase endo alpha; OTTMUSP0000000508; RP23-424K9.1.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 细胞生物  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Cat, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量 54kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MANEA:61-160/462 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain. Post-translational modifications: Undergoes proteolytic cleavage in the C-terminal region. Similarity: Belongs to the glycosyl hydrolase 99 family. SWISS: Q5SRI9 Gene ID: 79694 Database links:

Entrez Gene: 79694 Human

Entrez Gene: 242362 Mouse

Entrez Gene: 140808 Rat

Omim: 612327 Human

SwissProt: Q5SRI9 Human

SwissProt: Q6NXH2 Mouse

SwissProt: Q5GF25 Rat

Unigene: 533323 Human

Unigene: 245602 Mouse

Unigene: 10855 Rat

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.   
产品图片
Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MANEA) Polyclonal Antibody, Unconjugated (bs-18652R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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