货号: | YB-2779 |
生长状态: | 贴壁生长 |
年限: | 见说明书 |
运输方式: | 干冰运输or活细胞运输 |
器官来源: | 见说明书 |
是否是肿瘤细胞: | 见说明书 |
细胞形态: | 上皮样;多角形 |
免疫类型: | 请咨询销售人员 |
物种来源: | 人、大鼠、小鼠、、、、 |
组织来源: | 见说明书 |
ATCC Number: | 详见官方网站 |
细胞类型: | 复苏 |
供应商: | 钰博生物 |
数量: | 大量 |
Recombinant Human Formiminotransferase CyclodeaminaseSynonyms Formiminotransferase-cyclodeaminase, FTCD, LCHC1, LC-1.
Introduction Formiminotransferase cyclodeaminase is an enzyme which catalyzes the conversion of formiminoglutamateand tetrahydrofolateinto formiminotetrahydrofolateand glutamate.
Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker).
Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.
Recombinant Human Formiminotransferase CyclodeaminaseDescription Formiminotransferase Cyclodeaminase Human Recombinant (also called liver cytosol type 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 59,749 Dalton.
The FTCD is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Source Sf9 insect cells.
Formulation FTCD is supplied in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.
Recombinant Human Formiminotransferase CyclodeaminaseImmunological functions 1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).
Applications Western-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.
Coating concentration 0.35-0.7 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Storage Conditions Store at 4℃ if entire vial will be used within 2-4 weeks.
Store, frozen at -20℃for longer periods of time.
Avoid multiple freeze-thaw cycles.
Recombinant Human Formiminotransferase CyclodeaminasePurity Greater than 95% as determined by SDS-PAGE.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.