pET-28b-Cas9-His

载体基本信息

载体质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS pET-28b-Cas9-His Sequencing Result	9375 bp 	DNA	SYNDEFINITION pET-28b-Cas9-His Sequencing ResultACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..9375 /organism="pET-28b-Cas9-His Sequencing Result" /mol_type="other DNA" misc_feature 230..252 /label="pGEX_3_primer" misc_feature complement(246..437) /label="ROP" gene complement(909..1184) /label="tet (611 - 336)" /gene="tet (611 - 336)" misc_feature complement(1246..2337) /label="lacI" CDS complement(1246..2205) /label="ORF frame 1" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" CDS complement(2078..2578) /label="ORF frame 3" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" gene complement(2429..2680) /label="tet (887 - 636)" /gene="tet (887 - 636)" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" misc_feature 2612..2631 /label="pBRrevBam_primer" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" promoter 2715..2733 /label="T7_promoter" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" misc_feature 2733..2760 /label="lacO" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" misc_feature 2778..2794 /label="T7_transl_en_RBS" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV ANCRGD*" CDS complement(2784..3281) /label="ORF frame 2" /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH GISPS*" CDS 2802..6974 /label="ORF frame 3" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDSRADPKKKRK VAAALEHHHHHH*" CDS complement(4206..4724) /label="ORF frame 2" /translation="MSVSFQAFFNHLPIFKQRKGEDNVLKNVLVLLIVQEVLVFNDFQ EIVIRSQGCVEAILHSADFNRVETFNLFEIVFFELFHGNFPVRLEEEVHDSFLLLSRQ ECWLSHPFCDVFDLGELVINCEVLVQQRVFRKHLFVRQIFIKVSHPFDEGLGRGPLIH DFLEVPGSDGLF*" CDS complement(4788..5321) /label="ORF frame 2" /translation="MIHVVVGEPIDVQFLIHVHVPAVLQVVQVELLILKLGVFNWVFL KDLGPQFFYTLFNPLHPFPTVLLSLLGSLVLSGHLDNDILGLMPSHYFDEFIHDLNGL QYSLFDSWATCKISDVLVKTVPLARNLCFLDVLLKGERVIMDQLHEVPVGKSIGLKKI QDCLSTLLVSDPIDQFS*" CDS complement(6315..6848) /label="ORF frame 2" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6918..6938 /label="SV40 NLS" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6924..6938 /label="NLS" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature 6954..6971 /label="6xHIS" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" terminator 6982..7110 /label="T7_terminator" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" misc_feature complement(7024..7042) /label="T7_Terminal_primer" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" gene complement(7669..8484) /label="KanR2" /gene="KanR2" /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD LFISFRLEKVDGVFFEA*" CDS complement(7669..8484) /label="ORF frame 1" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" rep_origin 8590..9209 /label="pBR322_origin" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"ORIGIN 1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgCAATGGTG 61 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG 121 CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA 181 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC 241 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA 301 TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG 361 AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT 421 TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG 481 ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG 541 TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA 601 ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC 661 CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT 721 TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC 781 GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA 841 GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC 901 CGTGGGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA 961 CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG 1021 ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC 1081 ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG 1141 CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT 1201 CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC 1261 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC 1321 GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG 1381 ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC 1441 CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC 1501 GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT 1561 GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT 1621 GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC 1681 CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG 1741 CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA 1801 TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT 1861 GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC 1921 CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG 1981 CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA 2041 CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA 2101 CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC 2161 CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT 2221 TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA 2281 AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT TCACCACCCT 2341 GAATTGACTC TCTTCCGGGC GCTATCATGC CATACCGCGA AAGGTTTTGC GCCATTCGAT 2401 GGTGTCCGGG ATCTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA 2461 GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC 2521 CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA 2581 GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA 2641 CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT 2701 CGATCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC 2761 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC CATGGACAAG AAGTACTCCA 2821 TTGGGCTCGA TATCGGCACA AACAGCGTCG GCTGGGCCGT CATTACGGAC GAGTACAAGG 2881 TGCCGAGCAA AAAATTCAAA GTTCTGGGCA ATACCGATCG CCACAGCATA AAGAAGAACC 2941 TCATTGGCGC CCTCCTGTTC GACTCCGGGG AGACGGCCGA AGCCACGCGG CTCAAAAGAA 3001 CAGCACGGCG CAGATATACC CGCAGAAAGA ATCGGATCTG CTACCTGCAG GAGATCTTTA 3061 GTAATGAGAT GGCTAAGGTG GATGACTCTT TCTTCCATAG GCTGGAGGAG TCCTTTTTGG 3121 TGGAGGAGGA TAAAAAGCAC GAGCGCCACC CAATCTTTGG CAATATCGTG GACGAGGTGG 3181 CGTACCATGA AAAGTACCCA ACCATATATC ATCTGAGGAA GAAGCTTGTA GACAGTACTG 3241 ATAAGGCTGA CTTGCGGTTG ATCTATCTCG CGCTGGCGCA TATGATCAAA TTTCGGGGAC 3301 ACTTCCTCAT CGAGGGGGAC CTGAACCCAG ACAACAGCGA TGTCGACAAA CTCTTTATCC 3361 AACTGGTTCA GACTTACAAT CAGCTTTTCG AAGAGAACCC GATCAACGCA TCCGGAGTTG 3421 ACGCCAAAGC AATCCTGAGC GCTAGGCTGT CCAAATCCCG GCGGCTCGAA AACCTCATCG 3481 CACAGCTCCC TGGGGAGAAG AAGAACGGCC TGTTTGGTAA TCTTATCGCC CTGTCACTCG 3541 GGCTGACCCC CAACTTTAAA TCTAACTTCG ACCTGGCCGA AGATGCCAAG CTTCAACTGA 3601 GCAAAGACAC CTACGATGAT GATCTCGACA ATCTGCTGGC CCAGATCGGC GACCAGTACG 3661 CAGACCTTTT TTTGGCGGCA AAGAACCTGT CAGACGCCAT TCTGCTGAGT GATATTCTGC 3721 GAGTGAACAC GGAGATCACC AAAGCTCCGC TGAGCGCTAG TATGATCAAG CGCTATGATG 3781 AGCACCACCA AGACTTGACT TTGCTGAAGG CCCTTGTCAG ACAGCAACTG CCTGAGAAGT 3841 ACAAGGAAAT TTTCTTCGAT CAGTCTAAAA ATGGCTACGC CGGATACATT GACGGCGGAG 3901 CAAGCCAGGA GGAATTTTAC AAATTTATTA AGCCCATCTT GGAAAAAATG GACGGCACCG 3961 AGGAGCTGCT GGTAAAGCTT AACAGAGAAG ATCTGTTGCG CAAACAGCGC ACTTTCGACA 4021 ATGGAAGCAT CCCCCACCAG ATTCACCTGG GCGAACTGCA CGCTATCCTC AGGCGGCAAG 4081 AGGATTTCTA CCCCTTTTTG AAAGATAACA GGGAAAAGAT TGAGAAAATC CTCACATTTC 4141 GGATACCCTA CTATGTAGGC CCCCTCGCCC GGGGAAATTC CAGATTCGCG TGGATGACTC 4201 GCAAATCAGA AGAGACCATC ACTCCCTGGA ACTTCGAGGA AGTCGTGGAT AAGGGGGCCT 4261 CTGCCCAGTC CTTCATCGAA AGGATGACTA ACTTTGATAA AAATCTGCCT AACGAAAAGG 4321 TGCTTCCTAA ACACTCTCTG CTGTACGAGT ACTTCACAGT TTATAACGAG CTCACCAAGG 4381 TCAAATACGT CACAGAAGGG ATGAGAAAGC CAGCATTCCT GTCTGGAGAG CAGAAGAAAG 4441 CTATCGTGGA CCTCCTCTTC AAGACGAACC GGAAAGTTAC CGTGAAACAG CTCAAAGAAG 4501 ACTATTTCAA AAAGATTGAA TGTTTCGACT CTGTTGAAAT CAGCGGAGTG GAGGATCGCT 4561 TCAACGCATC CCTGGGAACG TATCACGATC TCCTGAAAAT CATTAAAGAC AAGGACTTCC 4621 TGGACAATGA GGAGAACGAG GACATTCTTG AGGACATTGT CCTCACCCTT ACGTTGTTTG 4681 AAGATAGGGA GATGATTGAA GAACGCTTGA AAACTTACGC TCATCTCTTC GACGACAAAG 4741 TCATGAAACA GCTCAAGAGG CGCCGATATA CAGGATGGGG GCGGCTGTCA AGAAAACTGA 4801 TCAATGGGAT CCGAGACAAG CAGAGTGGAA AGACAATCCT GGATTTTCTT AAGTCCGATG 4861 GATTTGCCAA CCGGAACTTC ATGCAGTTGA TCCATGATGA CTCTCTCACC TTTAAGGAGG 4921 ACATCCAGAA AGCACAAGTT TCTGGCCAGG GGGACAGTCT TCACGAGCAC ATCGCTAATC 4981 TTGCAGGTAG CCCAGCTATC AAAAAGGGAA TACTGCAGAC CGTTAAGGTC GTGGATGAAC 5041 TCGTCAAAGT AATGGGAAGG CATAAGCCCG AGAATATCGT TATCGAGATG GCCCGAGAGA 5101 ACCAAACTAC CCAGAAGGGA CAGAAGAACA GTAGGGAAAG GATGAAGAGG ATTGAAGAGG 5161 GTATAAAAGA ACTGGGGTCC CAAATCCTTA AGGAACACCC AGTTGAAAAC ACCCAGCTTC 5221 AGAATGAGAA GCTCTACCTG TACTACCTGC AGAACGGCAG GGACATGTAC GTGGATCAGG 5281 AACTGGACAT CAATCGGCTC TCCGACTACG ACGTGGATCA TATCGTGCCC CAGTCTTTTC 5341 TCAAAGATGA TTCTATTGAT AATAAAGTGT TGACAAGATC CGATAAAAAT AGAGGGAAGA 5401 GTGATAACGT CCCCTCAGAA GAAGTTGTCA AGAAAATGAA AAATTATTGG CGGCAGCTGC 5461 TGAACGCCAA ACTGATCACA CAACGGAAGT TCGATAATCT GACTAAGGCT GAACGAGGTG 5521 GCCTGTCTGA GTTGGATAAA GCCGGCTTCA TCAAAAGGCA GCTTGTTGAG ACACGCCAGA 5581 TCACCAAGCA CGTGGCCCAA ATTCTCGATT CACGCATGAA CACCAAGTAC GATGAAAATG 5641 ACAAACTGAT TCGAGAGGTG AAAGTTATTA CTCTGAAGTC TAAGCTGGTC TCAGATTTCA 5701 GAAAGGACTT TCAGTTTTAT AAGGTGAGAG AGATCAACAA TTACCACCAT GCGCATGATG 5761 CCTACCTGAA TGCAGTGGTA GGCACTGCAC TTATCAAAAA ATATCCCAAG CTTGAATCTG 5821 AATTTGTTTA CGGAGACTAT AAAGTGTACG ATGTTAGGAA AATGATCGCA AAGTCTGAGC 5881 AGGAAATAGG CAAGGCCACC GCTAAGTACT TCTTTTACAG CAATATTATG AATTTTTTCA 5941 AGACCGAGAT TACACTGGCC AATGGAGAGA TTCGGAAGCG ACCACTTATC GAAACAAACG 6001 GAGAAACAGG AGAAATCGTG TGGGACAAGG GTAGGGATTT CGCGACAGTC CGGAAGGTCC 6061 TGTCCATGCC GCAGGTGAAC ATCGTTAAAA AGACCGAAGT ACAGACCGGA GGCTTCTCCA 6121 AGGAAAGTAT CCTCCCGAAA AGGAACAGCG ACAAGCTGAT CGCACGCAAA AAAGATTGGG 6181 ACCCCAAGAA ATACGGCGGA TTCGATTCTC CTACAGTCGC TTACAGTGTA CTGGTTGTGG 6241 CCAAAGTGGA GAAAGGGAAG TCTAAAAAAC TCAAAAGCGT CAAGGAACTG CTGGGCATCA 6301 CAATCATGGA GCGATCAAGC TTCGAAAAAA ACCCCATCGA CTTTCTCGAG GCGAAAGGAT 6361 ATAAAGAGGT CAAAAAAGAC CTCATCATTA AGCTTCCCAA GTACTCTCTC TTTGAGCTTG 6421 AAAACGGCCG GAAACGAATG CTCGCTAGTG CGGGCGAGCT GCAGAAAGGT AACGAGCTGG 6481 CACTGCCCTC TAAATACGTT AATTTCTTGT ATCTGGCCAG CCACTATGAA AAGCTCAAAG 6541 GGTCTCCCGA AGATAATGAG CAGAAGCAGC TGTTCGTGGA ACAACACAAA CACTACCTTG 6601 ATGAGATCAT CGAGCAAATA AGCGAATTCT CCAAAAGAGT GATCCTCGCC GACGCTAACC 6661 TCGATAAGGT GCTTTCTGCT TACAATAAGC ACAGGGATAA GCCCATCAGG GAGCAGGCAG 6721 AAAACATTAT CCACTTGTTT ACTCTGACCA ACTTGGGCGC GCCTGCAGCC TTCAAGTACT 6781 TCGACACCAC CATAGACAGA AAGCGGTACA CCTCTACAAA GGAGGTCCTG GACGCCACAC 6841 TGATTCATCA GTCAATTACG GGGCTCTATG AAACAAGAAT CGACCTCTCT CAGCTCGGTG 6901 GAGACAGCAG GGCTGACCCC AAGAAGAAGA GGAAGGTGGC GGCCGCACTC GAGCACCACC 6961 ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG 7021 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT 7081 TTTTGCTGAA AGGAGGAACT ATATCCGGAT TGGCGAATGG GACGCGCCCT GTAGCGGCGC 7141 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 7201 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 7261 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 7321 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 7381 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 7441 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 7501 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 7561 ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 7621 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA ATTAATTCTT AGAAAAACTC 7681 ATCGAGCATC AAATGAAACT GCAATTTATT CATATCAGGA TTATCAATAC CATATTTTTG 7741 AAAAAGCCGT TTCTGTAATG AAGGAGAAAA CTCACCGAGG CAGTTCCATA GGATGGCAAG 7801 ATCCTGGTAT CGGTCTGCGA TTCCGACTCG TCCAACATCA ATACAACCTA TTAATTTCCC 7861 CTCGTCAAAA ATAAGGTTAT CAAGTGAGAA ATCACCATGA GTGACGACTG AATCCGGTGA 7921 GAATGGCAAA AGTTTATGCA TTTCTTTCCA GACTTGTTCA ACAGGCCAGC CATTACGCTC 7981 GTCATCAAAA TCACTCGCAT CAACCAAACC GTTATTCATT CGTGATTGCG CCTGAGCGAG 8041 ACGAAATACG CGATCGCTGT TAAAAGGACA ATTACAAACA GGAATCGAAT GCAACCGGCG 8101 CAGGAACACT GCCAGCGCAT CAACAATATT TTCACCTGAA TCAGGATATT CTTCTAATAC 8161 CTGGAATGCT GTTTTCCCGG GGATCGCAGT GGTGAGTAAC CATGCATCAT CAGGAGTACG 8221 GATAAAATGC TTGATGGTCG GAAGAGGCAT AAATTCCGTC AGCCAGTTTA GTCTGACCAT 8281 CTCATCTGTA ACATCATTGG CAACGCTACC TTTGCCATGT TTCAGAAACA ACTCTGGCGC 8341 ATCGGGCTTC CCATACAATC GATAGATTGT CGCACCTGAT TGCCCGACAT TATCGCGAGC 8401 CCATTTATAC CCATATAAAT CAGCATCCAT GTTGGAATTT AATCGCGGCC TAGAGCAAGA 8461 CGTTTCCCGT TGAATATGGC TCATAACACC CCTTGTATTA CTGTTTATGT AAGCAGACAG 8521 TTTTATTGTT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 8581 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 8641 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 8701 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 8761 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 8821 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 8881 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 8941 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT 9001 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 9061 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 9121 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 9181 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 9241 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 9301 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 9361 GCGAGGAAGC GGAAG