pCMV-SPORT-βgal

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载体质粒图谱和多克隆位点信息

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载体序列

LOCUS Exported 7854 bp ds-DNA circular SYN 12-AUG-2016KEYWORDS pCMV-SPORT-beta-galSOURCE synthetic DNA construct ORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7854) AUTHORS Biofeng Lab.FEATURES Location/Qualifiers source 1..7854 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 124..258 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /note="color: #a6acb3" promoter 645..663 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" /note="color: #ffffff; direction: RIGHT" CDS complement(1006..4053) /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /note="lacZ" /note="color: #993366" /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAELQLSAGRYHYQLVWCQK" promoter complement(4260..4278) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" /note="color: #ffffff; direction: LEFT" promoter complement(4394..4597) /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" /note="color: #ffffff; direction: LEFT" enhancer 4598..4901 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /note="color: #ffffff" protein_bind 4931..4947 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /note="color: #31849b" rep_origin complement(5449..6037) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" protein_bind 6115..6148 /bound_moiety="Cre recombinase" /note="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." /note="color: #31849b; direction: RIGHT" CDS complement(6250..7110) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 6250 .. 7041 / #ccffcc 2: 7042 .. 7110 / #ccffcc / signal sequence Cleavage site after base 7041" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin complement(7398..7853) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /note="color: #ffff00"ORIGIN 1 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 61 ttacgccagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg 121 atcgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 181 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 241 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 301 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat 361 gatcatgaac agactgtgag gactgagggg cctgaaatga gccttgggac tgtgaatcta 421 aaatacacaa acaattagaa tcactagctc ctgtgtataa tattttcata aatcatactc 481 agtaagcaaa actctcaagc agcaagcata tgcagctagt ttaacacatt atacacttaa 541 aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac 601 accacagaag taaggttcct tcacaaagat cccaagctag cttataatac gactcactat 661 agggagagag ctatgacgtc gcatgcacgc gtaagcttgg gcccctcgag ggatcctcta 721 gagcggccgc ctagagtcga ggccgagttt gtcagaaagc agaccaaaca gcggttggaa 781 taatagcgag aacagagaaa tagcggcaaa aataataccc gtatcacttt tgctgatatg 841 gttgatgtca tgtagccaaa tcgggaaaaa cgggaagtag gctcccatga taaaaaagta 901 aaagaaaaag aataaaccga acatccaaaa gtttgtgttt tttaaatagt acataatgga 961 tttccttacg cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca 1021 gaccaactgg taatggtagc gaccggcgct cagctgtaat tccgccgata ctgacgggct 1081 ccaggagtcg tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc 1141 ttccgcgtgc agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct 1201 gatgttgaac tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc 1261 gcagcgcaga ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag 1321 atcccagcgg tcaaaacagg cggcagtaag gcggtcggga tagttttctt gcggccctaa 1381 tccgagccag tttacccgct ctgctacctg cgccagctgg cagttcaggc caatccgcgc 1441 cggatgcggt gtatcgctcg ccacttcaac atcaacggta atcgccattt gaccactacc 1501 atcaatccgg taggttttcc ggctgataaa taaggttttc ccctgatgct gccacgcgtg 1561 agcggtcgta atcagcaccg catcagcaag tgtatctgcc gtgcactgca acaacgctgc 1621 ttcggcctgg taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg 1681 ggtcgcttca cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag 1741 cggcgtcagc agttgttttt tatcgccaat ccacatctgt gaaagaaagc ctgactggcg 1801 gttaaattgc caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag 1861 atgcgggatg gcgtgggacg cggcggggag cgtcacactg aggttttccg ccagacgcca 1921 ctgctgccag gcgctgatgt gcccggcttc tgaccatgcg gtcgcgttcg gttgcactac 1981 gcgtactgtg agccagagtt gcccggcgct ctccggctgc ggtagttcag gcagttcaat 2041 caactgttta ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttacc 2101 atccagcgcc accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct 2161 ggtcacttcg atggtttgcc cggataaacg gaactggaaa aactgctgct ggtgttttgc 2221 ttccgtcagc gctggatgcg gcgtgcggtc ggcaaagacc agaccgttca tacagaactg 2281 gcgatcgttc ggcgtatcgc caaaatcacc gccgtaagcc gaccacgggt tgccgttttc 2341 atcatattta atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg 2401 atactgacga aacgcctgcc agtatttagc gaaaccgcca agactgttac ccatcgcgtg 2461 ggcgtattcg caaaggatca gcgggcgcgt ctctccaggt agcgaaagcc attttttgat 2521 ggaccatttc ggcacagccg ggaagggctg gtcttcatcc acgcgcgcgt acatcgggca 2581 aataatatcg gtggccgtgg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg 2641 atcgacagat ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgattcatt 2701 ccccagcgac cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac 2761 gcgttcgctc atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat 2821 gccgtgggtt tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt 2881 gtaccacagc ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt 2941 catcagcagg atatcctgca ccatcgtctg ctcatccatg acctgaccat gcagaggatg 3001 atgctcgtga cggttaacgc ctcgaatcag caacggcttg ccgttcagca gcagcagacc 3061 attttcaatc cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc 3121 gtcggcggtg tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag 3181 tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg gcataaccac cacgctcatc 3241 gataatttca ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa 3301 agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag 3361 tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt 3421 agtcggttta tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg 3481 atcttccaga taactgccgt cactccaacg cagcaccatc accgcgaggc ggttttctcc 3541 ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa cgactgtcct ggccgtaacc 3601 gacccagcgc ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg 3661 cgtctggcct tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt 3721 cggattctcc gtgggaacaa acggcggatt gaccgtaatg ggataggtta cgttggtgta 3781 gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc 3841 aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa 3901 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 3961 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 4021 agggttttcc cagtcacgac gttgtaaaac gacgggatcg cgcttgagca gctccttgct 4081 ggtgtccaga ccaatgcctc ccagaccggc aacgaaaatc acgttcttgt tggtcaaagt 4141 aaacgacatg gtgacttctt ttttgcttta gcaggctctt tcgatccccg ggaattgcgg 4201 ccgccgacta gtgagctcgt cgacccggga attccggacc ggtacctgca ggcgtacctt 4261 ctatagtgtc acctaaatag ctttttgcaa aagcctaggc tagagtccgg aggctggatc 4321 ggtcccggtg tcttctatgg aggtcaaaac agcgtggatg gcgtctccag gcgatctgac 4381 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt 4441 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa 4501 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct 4561 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata 4621 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg 4681 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat 4741 gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc 4801 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg 4861 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gacctgcacg atgctgtttc 4921 ctgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgga agctataaag 4981 tgtaaagcct ggggtgccta atgagtgaaa gggcctcgta tcacgcctat ttttataggt 5041 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 5101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 5161 ataaccctga taaatgcttc aataatattg aaaaacgcgc gaattgcaag ctctgcatta 5221 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 5281 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 5341 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 5401 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 5461 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 5521 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 5581 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 5641 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 5701 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 5761 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 5821 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 5881 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 5941 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6001 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6061 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgccataact 6121 tcgtatagca tacattatac gaagttatgg catgagatta tcaaaaagga tcttcaccta 6181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 6241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 6601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 6901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 7021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7141 ttatcagggt tattgtctca tgccaggggt gggcacacat atttgatacc agcgatccct 7201 acacagcaca taattcaatg cgacttccct ctatcgcaca tcttagacct ttattctccc 7261 tccagcacac atcgaagctg ccgagcaagc cgttctcacc agtccaagac ctggcatgag 7321 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 7381 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt 7441 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 7501 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 7561 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 7621 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 7681 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 7741 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 7801 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtc