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Donkeyanti-Goat IgG 
Akt (pan) (40D4) Mou
Donkey Anti-Rabbit I
蛋白质质谱鉴定服务
0.2ml透明半裙边96孔P
AXYGEN耗材200ul枪头
康宁50ml灭菌离心管
AXYGEN耗材1000ul枪头
p38α MAPK (L53F8) Mouse mAb #9228
| 9228S | 100 ul ( 10 western blots ) | |
| 9228 |
| W | 1:1000 | Human, Mouse, Rat, Monkey, Pig,S. cerevisiae | Endogenous | 40 | Mouse IgG1 |
| IF-IC | 1:25 | ||||
| F | 1:200 |
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
p38α MAP Kinase (L53F8) Mouse mAb detects endogenous levels of total p38α MAPK. This antibody does not cross-react with either JNK/SAPK or p42/44 MAPK or other isoforms of p38.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant p38 MAPK protein.
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p38α MAPK (L53F8) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p38α MAPK (L53F8) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using p38-alpha MAPK (L53F8) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
- Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.
- Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.
Application References
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Companion Products
- 9913 Phospho-p38 MAPK Pathway Sampler Kit
- 9213 p38 MAPK Control Cell Extracts
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- 2371 p38α MAPK (7D6) Rabbit mAb
- 9217 p38α MAPK (5F11) Mouse mAb
- 2339 p38β MAPK (C28C2) Rabbit mAb
- 2308 p38δ MAPK (10A8) Rabbit mAb
- 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
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- 2307 p38γ MAPK Antibody
- 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
- 9212 p38 MAPK Antibody
- 9218 p38α MAPK Antibody
- 9214 p38δ MAPK Antibody
- 9210 PhosphoPlus p38 MAPK (Thr180/Tyr182) Antibody Kit
- 9219 Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose Bead Conjugate)
- 7072 Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO Reagent and 20X Peroxide
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