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Anti-Clumping Agent

产品价格: ¥785.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2019-06-11 16:44:11
浏览次数:
22

产品详情

品牌名称:
Gibco
货号: 0010057AE
CAS号: 细胞解离试剂
供应商: 恒斐生物
数量: 大量
英文名: Anti-Clumping Agent
保存条件: 2-8度
规格: 20ml

描述

GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.

• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin free (AOF) and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery

Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping" state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly. 

Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield. 

For actual cell dissociation, we recommend our animal origin-free TrypLE™ products

Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection. 

Animal Origin Free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch. 

Convenient and Customizable Packaging
In addition to this 20 ml size, Anti-Clumping Agent is also available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking the "Customize this Product" link under the "Add to Cart" button above or by contacting us at CustomMedia@lifetech.com

Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Servicesgroup. 

规格

Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Reagent Type: Clumping Prevention Reagent
Form: Liquid
Format: Bottle(s)
Cell Line: CHO, 293 (HEK), Insect Cells
Culture Type: Suspension Cell Culture
Product Size: 20 mL
Volume (Metric): 20 ml
Shipping Condition: Room Temperature

内容及储存

Store at 2 to 8°C
温馨提示:不可用于临床治疗。

免责声明

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