人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒描述:
ScienCells GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:
- Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
- Signaling mediation: IGF1R, BMP2K, PROM1
- Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
- Mutated transcription factors identified
- in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
- in precursor B lymphoblastic leukemia: TCF3, PBX1
- in precursor T lymphoblastic leukemia: TLX1, TLX3
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*
(8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | AFF1 | CCND2 | CDKN1A | DUSP6 | HDAC1 | HPGD | KLF3 | MEIS1 | PBX1 | RUNX2 | TCF3 | β-actin |
B | ATM | CCNT1 | CDKN2C | ETV6 | HDAC2 | IGF1 | KMT2A | MLLT1 | PER2 | SIN3A | TLX1 | GAPDH |
C | BCL2A1 | CCNT2 | CEBPA | ETV7 | HHEX | IGF1R | LDB1 | MLLT3 | PROM1 | SIX1 | TLX3 | LDHA |
D | BCL2L1 | CCR7 | CSF1R | EYA1 | HIST2H3C | IL3 | LMO2 | MYC | PTCRA | SIX4 | TRAF1 | NONO |
E | BCL6 | CD14 | CSF2 | FCGR1A | HMGA2 | ITGB7 | LYL1 | MYCN | RARA | SMAD1 | UTY | PPIH |
F | BIRC3 | CD86 | DDIT3 | FUT8 | HOXA10 | JMJD1C | MAF | NCOR1 | REL | SP1 | WHSC1 | GDC |
G | BMP2K | CDK14 | DEFA3 | GRIA3 | HOXA11 | JUP | MAX | NFKB1 | RUNX1 | SPI1 | WNT16 | PPC |
H | CCNA1 | CDK9 | DOT1L | GZMB | HOXA9 | KDM6A | MEF2C | PAX5 | RUNX1T1 | SUPT3H | ZBTB16 | NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK030 |
---|---|
Country of Manufacture | United States |
Product Code | GQH-TMH |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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