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碱性磷酸酶(Sigma代理)P0114-10KU
重组人单核细胞趋化蛋白 MCP-2/CCL8
牛病毒性腹泻E2抗原BVDV重组抗原
重组人FGFB蛋白
Rnase H
重组人c-Myc-polyR蛋
重组人血小板因子4 
重组人巨噬细胞炎性蛋
重组人γ-干扰诱生蛋
重组人白介素1F5| 目录号 | 规格 | 价格 | 说明书下载 |
| C417A | 2μg | 400元 |  |
| C417B | 10μg | 1800元 | |
| C417C | 50μg | 4500元 |
Macrophage colony-stimulating factor 1, also known as M-CSF,CSF-1, Lanimostim, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. M-CSF is a cytokine and acts as a primary regulator of macrophage survival, proliferation and differentiation. It is also essential for the survival and proliferation of osteoclast progenitors. M-CSF plays important roles in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Recombinant M-CSF containing N terminal portion of M-CSF is sufficient for interaction with the M-CSF receptor. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species specific.
·DescriptionRecombinant Human M-CSF produced by transiently transfected human cell consists of sequence (Accession#P09603)(Glu33-Arg255) of human M-CSF fused with a polyhistidine tag at the C-terminus.
·Quality Control
Biological activity:The ED50 of rHuM-CSF is 0.5-1.5 ng/mL,as measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells.
Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino-Acid Sequence: Predicted N terminal amino acid is Glu33.
Endotoxin:Less than 0.1ng/μg (1EU/μg) of rHuM-CSF.
·FormulationSterile and Lyophilized from a 0.2 μm filtered solution in PBS.
·ReconstitutionIt is recommended to reconstitute the lyophilized rHuM-CSF in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
·Storage Lyophilized rHuM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuM-CSF should be stored at 4°C between 2-7 days and aliquots of reconstituted samples containing 0.1% BSA are stable up to 3 months below -18°C for future use.
