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3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)

产品价格: ¥580.00

最小起订量:暂无 可售数量:暂无

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所在地区:
全国
有效期至:
长期有效
最后更新:
2021-04-27 01:30:01
浏览次数:
325

产品详情

品牌名称:
HZbscience

 Mouse Anti-GAPDH(3E12)-Loading Control antibody

 

产品编号 hz-0978M
英文名称 GAPDH(3E12)-Loading Control
中文名称 3-***酸甘油醛脱氢酶单克隆抗体(内参抗体)
别    名 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10.  
   
产品类型 内参抗体 
研究领域 肿瘤  免疫学  神经生物学  信号转导  通道蛋白  新陈代谢  Alzheimers  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 3E12
交叉反应 Human, Mouse, Rat, Rabbit, 
产品应用 WB=1:500-5000 ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量 38kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Full length GAPDH protein purified from rabbit muscle: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background: Loading Control
产品图片
Sample:  Placenta (Mouse) Lysate at 40 ug 293T Cell Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD
Sample: Bladder(Rat)Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD
Sample:Liver(Mouse)Lysate at 40 ug Primary: Anti-GAPDH(bsm-0978M)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38kD Observed band size: 38kD
Sample:  Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GAPDH(3E12)-Loading Control (bsm-0978M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH(3E12)-Loading Control) Polyclonal Antibody, Unconjugated (bsm-0978M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB stainin

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