货号: | IVGN2404 |
CAS号: | 相关应用: 限制性内切酶和克隆酶 |
供应商: | 恒斐生物 |
数量: | 现货 |
英文名: | Anza DNA Blunt End Kit |
保存条件: | Store at -5 to -30°C. |
规格: | 100 reactions |
Anza DNA Blunt End Kit描述
The Invitrogen™ Anza™ DNA Blunt End Kit is used to convert DNA with cohesive ends to DNA with blunt ends for use in blunt-end ligation reactions. The included Anza™ DNA Blunting Enzyme Mix contains T4 DNA polymerase and Klenow Fragment, while the Anza™ 10X Blunting Buffer contains dNTPs to facilitate the synthesis of blunt ends. Use this kit when the desired vector does not contain a recognition site that would produce blunt ends.Features:
• Fast 15-minute, room-temperature protocol
• DNA digested with Anza™ restriction enzymes can be used directly in the Anza Blunt End protocol following heat inactivation
The Anza DNA Blunt End Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include theAnza T4 DNA Ligase Master Mix, Anza T4 PNK Kit, and Anza DNA End Repair Kit.
Anza DNA Blunt End Kit规格
Enzyme: | Klenow Fragment, T4 DNA polymerase |
---|---|
Product Size: | 100 reactions |
Shipping Condition: | Approved for shipment on Wet or Dry Ice |
Anza DNA Blunt End Kit内容及储存
200 µL Anza 10X Blunting Buffer
Store at -5 to -30°C.