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BD流式抗体564971Ms Caspase-11 Pure
DRP1 (Phospho-Ser616) Antibody
7005 Anti-type II collagen (CB11) a
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0.2ml PCR 单管， 平| 货号： | A-A-636-1000 |
| 抗体名： | 半乳糖凝集素-3抗体 |
| 抗体英文名： | Galectin-3 |
| 宿主： | 兔 |
| 供应商： | 倍诺博生物 |
| 克隆性： | 是 |
Source
Rabbit
Immunogen
Human Galectin-3
Clonality
Monoclonal Antibody
Applications
ELISA, CLIA, FIA, etc.
SPECIFICATIONS
Pairing antibody
Anti-Human Galectin-3 Capture antibody
Specificity
React to Human Galectin-3
Purity
>99%
Formulation
PBS, pH 7.0
Unit
1mg/mL
PREPARATION AND STORAGE
Shipping
This antibody is shipped at 4 oC.
Stability & Storage
This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20 oC to -70 oC. Avoid freeze-thaw cycles.
ACTIVITY ASSAY PROTOCOL
Principles
·Anti-Human Galectin-3 assay is a microtiter plate-based immunoassay for the quantitative determination of galectin-3 levels in human serum and plasma.
·Anti-Human Galectin-3 assay utilizes two monoclonal antibodies against galectin-3. The first monoclonal anti-human galectin-3 antibody is coated onto the surface of the wells in a microtiter plate and serves as the capture antibody to bind galectin-3 molecules in samples, while the other rabbit monoclonal anti-human galectin-3 antibody is provided in solution and functions as the tracer antibody for detecting galectin-3 molecules bound to the capture antibody.
· BNB provides ready-to-use microtiter plates and a monoclonal HRP-conjugated anti-galectin-3 antibody to support ELISA assay with procedures described below. Meanwhile, BNB offers other customized labeling on our tracer anti-galectin-3 for the purposes of FIA (Fluorescent Immunoassay) and CLIA (Chemiluminescent Immunoassays) that could be monitored at various wavelengths.
Test Procedure
1. Standards, quality control materials, and patient specimens are introduced in duplicate into the wells and incubated for 60 minutes. During this incubation, the galectin-3 present in the standards and specimens binds to the capture antibody coated onto the well surface. Subsequent washing removes all unbound material introduced with the samples, including unbound galectin-3.
2. The tracer antibody, a horseradish peroxidase (HRP)-labeled anti-galectin-3 antibody, is then introduced into the well and incubated for 60 minutes. During this time, an antibody-antigen-antibody complex is formed.
3. After a wash step to remove any unbound tracer antibody, the tetramethylbenzidine (TMB) substrate is added, yielding a blue color in the presence of HRP. The color development is stopped after 20 minutes by the addition of sulfuric acid, changing the color to yellow, which is read at an absorbance of 450 nm.
4. The absorbance is proportional to the galectin-3 levels in the specimens. The test results of the specimens are read from the standard curve.
DATA
Representative Standard Curve
Measuring Range
The Anti-Human Galectin-3 measuring range is 1.4 to 94.8 ng/mL with clinical specimens.
BACKGROUND & APPLICATION
Galectin-3 is a structurally unique member of a family of beta-galactoside-binding lectins. Expression of galectin-3 has been associated with the epithelium and inflammatory cells including macrophages, neutrophils and mast cells. Galectin-3 has been implicated in a variety of biological processes important in heart failure including myofibroblast proliferation, fibrogenesis, tissue repair, cardiac remodeling and inflammation.
联系方式：倍诺博生物 谷小姐 13632887329 0755-86966133
温馨提示：不可用于临床治疗。
