货号: | GK032 |
CAS号: | 咨询销售 |
供应商: | 中乔新舟 |
数量: | 大量 |
英文名: | GQH-SLC |
保质期: | 6个月 |
保存条件: | -20度 |
注册证号: | 咨询销售 |
Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应
美国ScienCell研究实验室(www.sciencellonline.com)成立于1999年,公司总部位于美国加州的圣地亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发,在全球拥有众多客户。在国内销售15年来,很多老师应用其产品发表了高质量的SCI文章,且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质,深受广大科研工作者的信赖。
ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制,细胞纯度可达98%。其中包括21
种人体正常细胞系统,90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离,产品质量过硬。
确保了实验结果的重复性和连贯性。
请登录Sciencell公司官方网站(www.zqxzbio.com或www.sciencellonline.com)以确保购买正规公司产品
人小细胞肺癌定量PCR芯片试剂盒描述:
ScienCell's GeneQuery™ Human Small Cell Lung Cancer qPCR Array Kit (GQH-SLC) is designed to facilitate gene expression profiling of 88 key genes involved in small cell lung cancer (SCLC) carcinogenesis. SCLC accounts for approximately 10-20% of all lung cancers. Compared to non-small cell lung cancer (NSCLC), SCLC usually grows and metastasizes more rapidly. Brief examples of how included genes may be grouped according to their functions are shown below:
- Oncogenes and tumor suppressor genes: MYC, RARB, FHIT, TP53, PTEN
- Cell cycle regulation and apoptosis: CDK2, CDK4, CDK6, CCND1, CCNE2, E2F2, CASP9
- PI3K/AKT pathway: PI3Ks, AKT1, AKT2
- IKK/NFKB pathway: NFKB1, CHUK, IKBKB
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Small Cell Lung Cancer qPCR Array Plate Layout*
(8 controls in Bold and Italic)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | ABCB1 | CCND1 | CHUK | CSF3 | HTATIP2 | KRT19 | LAMB2 | MYCL | PIK3CA | PTK2 | SYP | β-actin |
B | ABCC1 | CCNE2 | CKS1B | CXCR2 | IKBKB | KRT20 | LAMB3 | NCAM1 | PIK3CD | RARB | TOP1 | GAPDH |
C | AKT1 | CDK2 | CKS2 | CYCS | ITGA2 | KRT7 | LAMB4 | NFKB1 | PIK3CG | RASSF1 | TP53 | LDHA |
D | AKT2 | CDK4 | COL4A1 | E2F2 | ITGA2B | LAMA1 | LAMC1 | NKX2-1 | PIK3R1 | RCVRN | TRAF1 | NONO |
E | APAF1 | CDK6 | COL4A2 | EGFR | ITGA3 | LAMA2 | LAMC2 | NMB | PIK3R3 | RXRA | TRAF4 | PPIH |
F | BIRC2 | CDKN1B | COL4A4 | ENO2 | ITGAV | LAMA3 | LAMC3 | NMBR | PIK3R5 | RXRG | TRAF5 | GDC |
G | BIRC7 | CDKN2A | COL4A5 | FHIT | ITGB1 | LAMA5 | MAX | NOS2 | PTEN | SKP2 | TRAF6 | PPC |
H | CASP9 | CHGA | COL4A6 | FN1 | KIT | LAMB1 | MYC | PIAS2 | PTGS2 | SST | XIAP | NTC |
* gene selection may be updated based on new research and development
Catalog No. | GK032 |
---|---|
Country of Manufacture | United States |
Product Code | GQH-SLC |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | GQH-SLC is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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