点击图片查看原图
改良的Lowry蛋白定量分析试剂
链霉亲和素琼脂糖凝胶FF（Streptavidi
蛇皮透析管
AN-029 Minute™ 脂肪
EI-027 Minute™ 高效
Minute TM 细胞质和细
AF-023 Minute™ 脂肪
SC-003 Minute™ 细胞
SM-005 Minute™质膜
CP-011 Minute™ 植物
MM-018 Minute™ 微粒| 货号： | SD-008/SN-009 |
| 英文名： | Minute TM Total Protein Extraction Kit for Plant Tissues |
| 保存条件： | RT |
| 规格： | 50tests |
MinuteTM 植物组织总蛋白提取试剂盒
目录号：SD-008/SN-009
描述
植物组织总蛋白提取试剂盒，由优化细胞裂解液和0.6 ml离心管柱组成，能够迅速从植物组织（叶片，种子，柔软的干和根等）中提取总蛋白。试剂盒同时提供天然和变性两种不同的裂解液，可供用户选择。离心管柱技术提取蛋白质，可从20-200mg植物组织中中提取总蛋白质，操作时间5-8分钟，得率可达2-8mg/ml。
应用
提取的蛋白质样品可以应用于SDS-PAGE, immunoblottings, ELISA, IP, ELISA，蛋白质分析和小规模蛋白层析纯化。
Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.
操作步骤
操作流程图
Protocol请点击产品资料下载查看
产品订购信息| Minute TM Total Protein Extraction Kit for Plant Tissues (50 Tests) | SD-008/SN-009 | 2875.00 |
| Minute TM Total Protein Extraction Kit for Plant Tissues (4Tests) | SD-008/SN-009 | 290.00 |
1. Crystal R. et al. (2013). Expression profiling of four defense-related buffalograss transcripts in response to chinch bug (hemiptera: Blissidae) feeding. Journal of Economic Entomology. 106 (6):2568-2576.
2. Weber, C. F. (2017). Microgreen Farming and Nutrition: A Discovery-Based Laboratory Module to Cultivate Biological and Information Literacy in Undergraduates. The American Biology Teacher, 79(5), 375-386.
3. Abdulazeez, M., Abubakar, S. M., & Mu’azzam, J. M. (2017). Isolation and Characterization of a Potential Angiotensin-Converting Enzyme Inhibitory Peptide from the Leaves of Leptadenia hastata (Asclepiadaceae). Malaysian Journal of Applied Sciences, 2(1), 35-47.
