货号: | SN-006 |
英文名: | Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues |
保存条件: | 4℃ |
规格: | 50tests |
MinuteTM 无表面活性剂的动物细胞/组织总蛋白提取试剂盒
目录号:SN-006
描述
无表面活性剂的动物细胞/组织总蛋白提取试剂盒,由优化细胞裂解液和0.6 ml离心管柱组成,能够迅速从动物细胞(昆虫/哺乳动物等)和组织(无脊椎动物和脊椎动物)中提取无表面活性剂的总蛋白。试剂盒中的裂解液不含任何表面活性剂和EDTA。离心管柱技术提取蛋白质,样品量最小可处理20ul,最大可达500ul。从细胞/组织中提取总蛋白质操作时间小于5分钟,得率可达1-5mg/ml。
应用提取的蛋白质样品可以应用于蛋白质组学(液相色谱/质谱),IP,ELISA,2D分析,等电聚焦,SDS-PAGE, immunoblottings等。
Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
操作步骤SN-006
操作流程图
Protocol
请点击产品资料下载查看
产品订购信息Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) | SN-006 | 2875.00 |
Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (4 Tests) | SN-006 | 290.00 |
速度快,操作简单
小样本量:最小可处理20ul
无蛋白丢失,高得率
无需超声处理,煮沸,或反复冻融
不含任何表面活性剂和EDTA
参考文献
1. Seo,J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002/adhm.201400173
2. M.Takada, S. Chiba et al. (2015). Inflammatory responses to neutral fatand fattyacids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International
http://dx.doi.org/10.1016/j.forsciint.2015.07.011
3. Ji-Hun Seo.et al(2016), Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039/C6RA03967G
4. Wenteng Xu.et al. Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), doi:10.1016/j.gene.2016.07.062