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泛素富集试剂盒
AT-022 Minute™ 总蛋白提取试剂盒（
蛋白A单结构域—EGFP融合蛋白（A-EGFP
AN-029 Minute™ 脂肪
EI-027 Minute™ 高效
Minute TM 细胞质和细
AF-023 Minute™ 脂肪
SC-003 Minute™ 细胞
SM-005 Minute™质膜
CP-011 Minute™ 植物
MM-018 Minute™ 微粒| 货号： | SN-006 |
| 英文名： | Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues |
| 保存条件： | 4℃ |
| 规格： | 50tests |
MinuteTM 无表面活性剂的动物细胞/组织总蛋白提取试剂盒
目录号：SN-006
描述
无表面活性剂的动物细胞/组织总蛋白提取试剂盒，由优化细胞裂解液和0.6 ml离心管柱组成，能够迅速从动物细胞（昆虫/哺乳动物等）和组织（无脊椎动物和脊椎动物）中提取无表面活性剂的总蛋白。试剂盒中的裂解液不含任何表面活性剂和EDTA。离心管柱技术提取蛋白质，样品量最小可处理20ul，最大可达500ul。从细胞/组织中提取总蛋白质操作时间小于5分钟，得率可达1-5mg/ml。
应用提取的蛋白质样品可以应用于蛋白质组学（液相色谱/质谱），IP，ELISA，2D分析，等电聚焦，SDS-PAGE, immunoblottings等。
Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
操作步骤
操作流程图
Protocol
请点击产品资料下载查看
产品订购信息| Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (50 Tests) | SN-006 | 2875.00 |
| Minute TM Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues (4 Tests) | SN-006 | 290.00 |
速度快，操作简单
小样本量：最小可处理20ul
无蛋白丢失，高得率
无需超声处理，煮沸，或反复冻融
不含任何表面活性剂和EDTA
参考文献
1. Seo,J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002/adhm.201400173
2. M.Takada, S. Chiba et al. (2015). Inflammatory responses to neutral fatand fattyacids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International
http://dx.doi.org/10.1016/j.forsciint.2015.07.011
3. Ji-Hun Seo.et al(2016), Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039/C6RA03967G
4. Wenteng Xu.et al. Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), doi:10.1016/j.gene.2016.07.062
