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P-reagent
组蛋白/DNA结合蛋白分离试剂盒
AN-029 Minute™ 脂肪
EI-027 Minute™ 高效
AF-023 Minute™ 脂肪
SC-003 Minute™ 细胞
SM-005 Minute™质膜
CP-011 Minute™ 植物
MM-018 Minute™ 微粒| 货号： | SC-003-4T |
| 规格： | 4tests |
MinuteTM 细胞质和细胞核分离试剂盒
目录号：SC-003
描述Minute 细胞质和细胞核分离试剂盒由细胞质提取缓冲液和细胞核提取缓冲液，离心管柱和2.0ML收集管组成。本试剂盒可快速从培养的哺乳动物细胞/组织，原生质体（植物，细菌，酵母和真菌）中分离天然的胞质和胞核蛋白，时间<15分钟。
应用本试剂盒可以快速分离天然的胞质和胞核蛋白，可应用于SDS-PAGE, immunoblottings, ELISA, IP，蛋白质定位，凝胶迁移分析，2-D等其他应用。
操作流程图
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产品订购信息
| Minute TM Cytoplasmic and Nuclear Fractionation kit (50 Tests) | SC-003 | 2875.00 |
| Minute TM Cytoplasmic and Nuclear Fractionation kit (4 Tests) | SC-003 | 290.00 |
1. Kalabus, JL. et al. (2012). MicroRNAs differentially regulate carbonyl reductase 1 (CBR1) gene expression dependent on the allele status of common polymorphic variant rs9024. PLOS ONE. 7(11):1-9.
2. Lombrafia AQ. et al (2014). Interindividual variability in the cardiac expression of anthracycline reductases in donors with and without down syndrome. Pharm Res. 31:1644-1655.
3. Hou, N., et al. (2014). Vitamin A deficiency impairs spatial learning and memory: The mechanism of abnormal CBP-dependent histone acetylation regulated by retinoic acid receptor alpha. Mol Neurobiol. DOI 10.1007/s12035-014-8741-6.
4. You, Y., et al. (2014). Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3. Cell Death and Disease. (2014)5, e1493; doi: 10.1038/cddis. 2014.443.
5. M Tanaka, et al（2016） Ex vivo effects of naphthoquinones on allergen-sensitized mononuclear cells in mice. International Journal of Immunopathology and Pharmacology 1-8; DOI: 10.1177/0394632016632221
6. Weilin Liu,et al (2016) Electroacupunctre improves motor impairment via inhibition of microgliamediated neuroinflammation in the sensorimotor cortex after ischemic stroke. Life Sciences. doi: 10.1016/j.lfs.2016.01.045
7. Yang Xu,et al (2016) RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF. Scientific Reports| 6:29362| DOI：10.1038/srep29362
8. Le Shi., et al (2016) Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-cateninmediated angiogenesis. Tumor Biol. DOI 10.1007/s13277-016-5251-3 9. Caleb Weihao Huang. Et al (2016). A novel slow-releasing hydrogen sulfide donor,FW1256,exerts anti-inflammatory effects in mouse macrophages and in vivo. Pharmacological Research. http://dx.doi.org/10.1016/j.phrs.2016.09.032
10. Yuichiro Matsuoka, et al. IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. British Journal of Cancer (2016), 1–11 | doi: 10.1038/bjc.2016.327
11. Hongli Hu, et al (2016). Acetylation of PGK1 Promotes Liver Cancer Cell Proliferation and Tumorigenesis. Hepatology. doi: 10.1002/hep.28887
12.Chao Meng., et al (2016). Lung inflation with hydrogen sulfide during the warm ischemia phase ameliorates injury in rat donor lungs via metabolic inhibition after cardiac death. Surgery. DOI: http://dx.doi.org/10.1016/j.surg.2016.10.031
