SM-005 Minute™质膜蛋白分离和细胞组分分离试剂盒

Minute^TM  质膜蛋白分离和细胞组分分离试剂盒

目录号：SM-005

      Minute ^TM 膜蛋白提取试剂盒通过离心管柱技术配合特定裂解液可以提取天然的膜蛋白。其原理是细胞/组织通过Buffer A致敏，然后细胞以Z字形路径通过离心管柱。在这个过程中细胞膜会破裂，分离出完整的细胞核，因此最终获得的膜蛋白中不含有核膜和核蛋白污染。通过差速离心和密度离心可以把细胞蛋白分为：总膜，细胞核，细胞质，细胞器及质膜，而无需超高速离心。不同于其他试剂盒，因为高速离心通过离心管柱时使细胞膜破裂，所以不需要匀浆，匀浆通常会引起膜蛋白提取结果的差异。而我们的试剂盒使用同样起始量的细胞，只需设定离心力和时间，结果一致性更好。整个操作过程不超过45分钟即可完成。

本试剂盒可以快速从细胞或组织中分离天然的膜蛋白，可应用于SDS-PAGE, immunoblottings, ELISA, IP,膜蛋白结构分析, 2-D,酶活性检测等其他应用。

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