点击图片查看原图

生物素定量检测试剂盒(比色法)

产品价格: ¥2775.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国北京
有效期至:
长期有效
最后更新:
2020-10-13 02:00:02
浏览次数:
27

产品详情

品牌名称:
biovision
货号: K811-100
保存条件: -20度
供应商: 北京博迈斯
英文名: Biotin Quantitation Kit (Colorimetric)
规格: 100 assays

Assay Kit Name:
Biotin Quantitation Kit (Colorimetric)

Kit Summary:
• Detection method - Absorbance (500 nm)
• Applications - Measuring free biotin or the substitution degree of Biotinylation (biotin labels) of proteins and antibodies

Features & Benefits:
• Simple & convenient
• This kit can be used to estimate as low as 60 pmol or 15 ng of biotin in solution

Kit Components:
• Biotin Assay Buffer
• Biotin Assay Reagent A
• Biotin Assay Reagent B
• Biotin Standard Solution (10 mM in DMSO)
• Biotinylated BSA (2 mg/ml)

Description:
Biotin (Vitamin H) is an important biomolecule that has been widely used in Biotinylation reactions to label antibodies or other proteins of interest. Due to its low molecular weight, biotin usually does not cause any significant changes in protein conformation and biological activity. Biotinylated proteins/antibodies are widely isolated and assayed with streptavidin pull-down assay, affinity chromatography, ELISA and Western blotting etc. Biovision offers a variety of ready-to-use Biotinylation reagents (Cat. # 2346, 2345, 2347, 2322, 2326, 2323). It is often desired to determine the degree of substitution of such biotinylated biomolecules. Biovision’s Biotin Assay Kit is based on the differential binding of Streptavidin to Biotin and a dye, 2-(4-hydroxyazobenzene) benzoic acid (HABA). When HABA is bound to Streptavidin, addition of free biotin or biotinylated biomolecules results in its displacement in the reaction. These changes are manifested in terms of decrease in the overall absorption (OD 500 nm) and can be quantitatively correlated to the amount of biotin present in solution. The kit also provides Biotin as a Standard and Biotinylated BSA as a positive control. This kit can be used to estimate as low as 60 pmol or 15 ng of biotin in solution.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

温馨提示:不可用于临床治疗。

免责声明

本网页所展示的有关【生物素定量检测试剂盒(比色法)】的信息/图片/参数等由的会员【北京博迈斯科技发展有限公司 】提供,由【生物实验采购网】会员【北京博迈斯科技发展有限公司 】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法 律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【生物素定量检测试剂盒(比色法)】有关的信息/图片/价格等及提供 【生物素定量检测试剂盒(比色法)】的商家公司简介、联系方式等信息。

在您的合法权益受到侵害时,请您致电,我们将竭诚为您服务,感谢您对【生物实验采购网】的关注与支持!

网站首页 | 关于我们 | 联系方式 | 使用协议 | 版权隐私 | 网站地图  |  排名推广  |  广告服务  |  积分换礼  |  网站留言  |  RSS订阅  |  违规举报
 
免责声明:本站有部分内容来自互联网,如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权,请来电或致函告之,本网站将在规定时间内给予删除等相关处理。