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重组人可溶性肿瘤坏死因子受体-1（Hum
Tyrphostin AG 1478
Sudan IV
[VIP第1年] 指数:2
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SimplyP总RNA提取试剂
二氢睾酮（双氢睾酮）
SLC2A4 Polyclonal An| 货号： | K249-50 |
| 供应商： | 北京博迈斯 |
| 英文名： | Microsome Isolation Kit |
| 保存条件： | -20度 |
| 规格： | 50 assays |
图一，微粒体结构示意图
常见的提取方法主要是靠超速离心法或者蔗糖密度梯度离心法，不仅费时费力，而且提取效果一般。为此，作为全球生命科学公司的领导者Biovision公司，为解决上述困境，精心研发而推出市场上唯一的微粒体分离提取试剂盒（K249-50）
微粒体（Microsome）分离试剂盒 有以下特点：
方法简便：专利配方和方案，可无需超速离心和蔗糖密度梯度离心
Assay Kit Name:
Microsome Isolation Kit
Kit Summary:
• Detection method -
• Applications - Convenient and fast isolation of microsomal fraction from animal tissues
- Assessment of CYP-mediated drug metabolism and xenobiotic biotransformation
- Protein profiling of microsomal membrane proteins by SDS-PAGE and Western blot
Sample Type:
• Mammalian glands and soft tissues such as liver, spleen, lungs etc.
• Cultured eukaryotic cell lines such as HepG2 human hepatic carcinoma cells
Features & Benefits:
• Simple & convenient protocol
• The kit provides unique formulations of ready-to-use buffers and reagents to isolate microsomes without the need for ultracentrifugation or sucrose gradient fractionation
Kit Components:
• Homogenization Buffer
• Storage Buffer
• Protease Inhibitor Cocktail
Description:
Microsomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily. Microsomal preparations are an affordable and convenient in vitro system for assessing Phase I biotransformation reactions, as they contain all of the xenobiotic-metabolizing CYP isozymes and the membrane-bound flavoenzymes (such as NADPH P450-Reductase and cytochrome b5) required for function of the multicomponent P450 enzyme system. BioVision’s Microsome Isolation Kit enables preparation of active microsomes in about one hour, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.
Storage Conditions:
-20°C
