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JM109 Competent Cells, >108cfu/µg
BCIP/TNBT Single Reagent, Blue/brow
羟基化多壁碳纳米管(短) 30-50 nm
[VIP第1年] 指数:1
Ezup柱式植物基因组DN| 货号： | B600580-0200 |
| 供应商： | 生工生物工程（上海）股份有限公司 |
| 数量： | 现货 |
| 英文名： | EcoICRI |
| 规格： | 200 U |
Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Recognition sites GAG↑CTC
Source Escherichia coli ICR
Assayed on Lambda DNA (HindIII-digest)
Optimal buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Optimal temperature 37°C
Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Methlation effect Methylation sensitivity not tested
Thermal inactivation enzyme is inactivated by incubation at 65°C for 20 mins.
Campatible Ends Ecl136II, Eco53kI, Psp124BI
, SacI
, SstI
温馨提示：不可用于临床治疗。
