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Kolliphor® P 188
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细胞爬片| 货号： | K431-40 |
| 供应商： | 上海研卉生物科技有限公司 |
| 英文名： | JNK Activity Assay Kit |
| 数量： | 大量 |
| 保存条件： | -20℃ |
| 规格： | 40次分析 |
Kit Summary:
• Detection method- Western blot analysis using a phospho-c-Jun specific antibody at 1:1000 dilutions
• Sample type- Cell and Tissue lysates
• Species reactivity- Mammalian
• Applications- The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody.
Features & Benefits:
• Simple procedure
• The kit is suitable for both research and drug discovery
• The kit specifically detects JNK, other kinase activity would not be detected
Kit components:
• Kinase Extraction Buffer
• JNK Specific Antibody
• Protein A Sepharose
• c-Jun Protein/ATP Mixture
• Kinase Assay Buffer
• Phospho-cJun Specific Antibody
Description:
c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity Immunoassay Kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. Activity of the JNK is then determined in a kinase reaction using recombinant c-Jun as substrate. Phosphorylation of the c-Jun can be analyzed by Western blot analysis using a phospho-c-Jun specific antibody. The kit specifically detects JNK, other kinase activity would not be detected.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.
