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细胞色素c凋亡检测试剂盒

产品价格: ¥6058.00

最小起订量:暂无 可售数量:999盒

发货时限:
暂无
所在地区:
中国上海
有效期至:
长期有效
最后更新:
2020-09-25 02:00:01
浏览次数:
47

产品详情

品牌名称:
Biovision
货号: K257-100
保存条件: -20℃
供应商: 上海研卉生物科技有限公司
数量: 大量
英文名: Cytochrome c Apoptosis Assay Kit
规格: KIT

Kit Summary:
• Detection method- Western blotting
• Sample type- Cells and Tissues
• Species reactivity- Mammalian
• Applications- Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.

Features & Benefits:
• Simple procedure; takes ~3 hours
• Fast and convenient
• Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.

Kit components:
• Mitochondria Extraction Buffer
• 5X Cytosol Extraction Buffer
• DTT (1 M)
• 500X Protease Inhibitor Cocktail
• Anti-Cytochrome c mouse mAb (0.2 mg/ml)

Description:
Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. BioVision’s Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.

温馨提示:不可用于临床治疗。

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