大鼠骨髓巨噬细胞(冻存细胞)，Rat Bone Marrow Macrophage (Frozen Cells)

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Product Description
Rat Bone Marrow Macrophages are derived from the tibias and femurs of 5 weeks old Sprague–Dawley Rats. Rat Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in Cell Biologics’ Culture Medium for 7-10 days. Each vial contains at least 8 x10⁶cells. Rat Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
Rat Bone Marrow Macrophages were tested for expression of markers using antibodies, CD11b by flow cytometry.
Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics will ship frozen cells on dry ice.  On receipt, immediately transfer frozen cells to liquid nitrogen until ready for experimental use.  Live-cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics Products
Rat Bone Marrow Macrophages from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent. Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Although Rat Bone Marrow Macrophages are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
 
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells with Cell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.