货号: | HZ-1101 |
英文名: | Recombinant Human FGF-8b |
规格: | 2 µg |
FGF-8b (Recombinant Human)
Product Description:
Human Cell Expressed | ||
Animal-derived product free | ||
Endotoxin-free* | ||
High Activity | ||
Authentic Glycosylation |
HumanKine® FGF-8b is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 30 to 45 kDa. This wide molecular mass is due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. The cytokine is greater than 95% pure.
Technical Specifications:
Species: Human | ||
Expression: Human Cell Expressed | ||
Activity: 15 to 60 ng/mL ED50 | ||
Purity: >95% | ||
Endotoxin: Endotoxin-free* | ||
Molecular Mass: 30 to 45 kDa monomer, glycosylated. |
Activity Assay:
The specific activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line.
Purity Confirmation:
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
*Endotoxin-free: <0.5 EU/µg
All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.