货号: | HZ-1164 |
英文名: | Recombinant Human IL-1 beta |
规格: | 10 µg |
IL-1 beta (Recombinant Human)
Product Description:
Human Cell Expressed | ||
Animal-derived product free | ||
Endotoxin-free* | ||
Very High Activity |
HumanKine® IL-1 beta expressed in human 293 cells has a very high activity with a typical ED50 of 0.02 to 0.08 ng/mL using a bioassay to measure stimulation of the proliferation of mouse D10S cells. In comparison with the E. coli expressed protein, IL-1 beta® is 50% more potent promoting human CD4+ T cell differentiation into Th17 cells. This cytokine is produced in a serum-free, chemically defined media.
Technical Specifications:
Species: Human | ||
Expression: Human Cell Expressed | ||
Activity: 0.02 to 0.08 ng/mL ED50 | ||
Purity: >95% | ||
Endotoxin: Endotoxin-free* | ||
Molecular Mass: 18 and 25 kDa, monomer, glycosylated |
Activity Assay:
The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.
Purity Confirmation:
The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
*Endotoxin-free: <0.5 EU/µg
All HumanKine proteins are expressed in the HumaXpress® human cell expression system and have low endotoxin levels with native human post-translational modifications and folding.