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DNP (AP) System
美国原装进口樱花SAKURA冷冻切片包埋
Mouse Ultrasensitive Insulin ELISA
[VIP第1年] 指数:2
通过静安区市场监管局认证 
Smad7 Antibody
SNTB1 Antibody
VEGFR1 (phospho Tyr1
M1-巨噬细胞培养基,化
人CD34+祖细胞,脐血,
Hepatocyte Maintenan| 货号： | 2020A |
| 供应商： | Nanoprobes |
| 保质期： | 1-2年 |
| 数量： | 99 |
| 保存条件： | -20℃ |
| 英文名： | Monomaleimido Nanogold In 5 aliquots |
| 规格： | 30 nmol |
Product Information
NANOGOLD® is a newly developed gold label, prepared using a discrete gold compound rather than a colloid.1 This kit contains the NANOGOLD® particle with a single maleimide functionality incorporated into a ligand on the surface of the gold particle; this has a specific reactivity towards sulfhydryl groups, and may be covalently linked to reduced disulfides in the hinge region of antibodies, as shown in the figure below (Figure 1). It may also be used to label cysteine residues in proteins, or other biomolecules with an accessible sulfhydryl group. Molecules which have been labeled using Monomaleimido-NANOGOLD® include proteins,2 peptides,3 and oligonucleotides.4 The reagent as supplied has been lyophilized from 0.02 M sodium phosphate at pH 6.5, with 150 mM sodium chloride; dissolution in 1 ml deionized water will produce a solution of activated NANOGOLD® in this buffer. NANOGOLD® conjugates can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. They are stable to wide ranges of pH and ionic strength, and are not radioactive or carcinogenic. The labeling reagent should be stored at -20°C.
Figure 1: Schematic showing NANOGOLD® labeling of Fab' fragment via reaction of a sulfhydryl and a maleimide group. 5 vials of reagent are supplied, each containing sufficient reagent to label up to 3 nmol of sulfhydryl groups; this corresponds to 0.15 mg of Fab' fragments, or 0.45 mg of IgG molecules.
