货号: | MT002 |
数量: | 99 |
保存条件: | 4℃ |
供应商: | MIAOTONG |
保质期: | 6-8周 |
英文名: | OX-LDL |
规格: | 2mg |
OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN
Catalog No: MT002
Lot No:
Quantity: 2.0 mg (milligrams) Protein/vial
Concentration: 2.0 mg/ml (Protein)
Packaging: Oxidized LDL is membrane filtered and aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4 and 5 uM EDTA-.NA2.
Preparation: MT Human LDL (Cat. No. MT001) (which was purified to Mmhomogeneity via ultra-centrifugation(1.019-1.063g/cc)) is oxidized using 5 Cu2SO4 (oxidant) in PBS at 37°C for 24 hours. Oxidation is terminated by adding excess EDTA. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 2.0fold further than the native LDL.
TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard.
Starting LDL 0.16 nmoles of MDA/mg Protein
Ox-LDL 25.2 nmoles of MDA/mg Protein
Storage &
Stability: This product is stable for 8 weeks after receipt when handled aseptically and stored at 2-8°C. NEVER FREEZE.
Special Note: After prolonged storage,some precipitate may be observed. This is normal for this product.Clarify out the aggregates by spinning in a microfuge for 2 minutes.
Biological Sample lots of MT Oxidized LDL are evaluated for receptor binding to
Activity: peritoneal macrophages in conjunction with our DiIOx-LDL and [I-125] Ox-LDL
*Preparations of Oxidized LDL are fairly unstable; plan your experiments in advance and use fresh material.
FOR RESEARCH USE ONLY