货号: | P7589 |
供应商: | 研卉生物 |
数量: | 3周 |
英文名: | Quant-iT™ PicoGreen® dsDNA Assay Kit |
规格: | KIT |
Quant-iT™ PicoGreen® dsDNA Assay Kit
描述
通过 PicoGreen® dsDNA 定量分析,可以在含有 ssDNA、RNA 和游离核苷酸的样品中检测低至 25 pg/ml 的 dsDNA (1)。 该分析在三个数量级范围内呈线性,并且具有较低的序列依赖性,因而可以准确测量多种来源的 DNA,包括基因组 DNA、病毒 DNA、小量提取 DNA 或 PCR 扩增产物。 PicoGreen® dsDNA 定量试剂 (PicoGreen® dsDNA Quantitation Reagent) 具有如下特点:
• 灵敏度比 UV 吸收读数高出几个数量级,节省了珍贵的样品(图 1)
• 在存在等摩尔量 RNA 的情况下,对 dsDNA 具有特异性(图 2)
• 易于使用-只需将染料添加到样品中,等待 5 分钟即可读取
• 适用于 96 孔和 384 孔板形式
• 与大多数荧光酶标仪、荧光计和荧光反应兼容
应用: PicoGreen® dsDNA 定量试剂 (PicoGreen® dsDNA Quantitation Reagent) 和试剂盒是 PCR 分析、样品芯片分析、DNA 损伤分析、酶活性分析、基因组 DNA 定量、复杂混合物中的 dsDNA 定量和病毒 DNA 定量的理想选择。
常用规格
Sample Type (General):
dsDNA
Platform:
Microplate Reader
Quantitation Range:
50 pg to 2 µg
Product Size:
1 mL
Shipping Condition:
Room Temperature
Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.
参考文献:
-
Cell cycle synchronization of Escherichia coli using the stringent response, with fluorescence labeling assays for DNA content and replication.
- 作者
- Ferullo DJ, Cooper DL, Moore HR, Lovett ST,
- 杂志
- Methods (2009) 48:8-13
- 产品用途
- (1) Flow cytometric analysis of E. coli DNA content. (2) Fluorescence microscopy analysis of E. coli size and septation (FM 4-64). (3) Measurement and visualization of newly replicated DNA in E. coli (EdU). proliferation, DNA synthesis, imaging, Click-iT Edu.
- ID:
- PN67515
-
Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain
- 作者
- Ikeda Y, Iwakiri S, Yoshimori T,
- 杂志
- J Pharm Biomed Anal (2009) 49:997-1002
- 产品用途
- Assay for quantitation of host cell DNA carryover in recombinant protein products
- ID:
- PN67674
-
Factors affecting quantification of total DNA by UV spectroscopy and PicoGreen fluorescence.
- 作者
- Holden MJ, Haynes RJ, Rabb SA, Satija N, Yang K, Blasic JR,
- 杂志
- J Agric Food Chem (2009) 57:7221-7226
- 产品用途
- Quantitation of DNA isolated from plant tissues (maize and soybean)
- ID:
- PN67675
-
Mitochondrial DNA is a direct target of anti-cancer anthracycline drugs.
- 作者
- Ashley N, Poulton J,
- 杂志
- Biochem Biophys Res Commun (2009) 378:450-455
- 产品用途
- Flow cytometric quantification of mitochondrial DNA (mtDNA) via selective quenching of nuclear PicoGreen dye fluorescence by doxorubicin.
- ID:
- PN67546
-
The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization.
- 作者
- He J, Mao CC, Reyes A, Sembongi H, Di Re M, Granycome C, Clippingdale AB, Fearnley IM, Harbour M, Robinson AJ, Reichelt S, Spelbrink JN, Walker JE, Holt IJ
- 杂志
- J Cell Biol (2007) 176:141-146
- ID:
- PN58836