增光霉素（博来霉素）别名：争光霉素Zeocin

Selection antibiotic: cell culture tested, sterile reagent

InvivoGen is the sole worldwide producer of Zeocin™, a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).

Resistance to Zeocin™ is conferred by the Sh ble gene product, which inactivates Zeocin™ by binding to the antibiotic. Sh ble is a small gene, only 370bp in size, carried in a number of InvivoGen’s vectors.

Zeocin™ is a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it. The action of Zeocin™ is effective on most aerobic cells.

Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.

Product concentration: 100 mg/ml in solution
CAS number: 11006-33-0

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level: < 1 EU/mg
Physicochemical characterization: HPLC, pH, appearance
Cell-culture tested: potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin™-resistant cells

Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer. This product is available in three pack sizes:
- ant-zn-1: 10 x 1 ml (1 g)
- ant-zn-5: 50 x 1 ml (5 g)
- ant-zn-5b: 1 x 50 ml (5 g)

Zeocin™ is also supplied as a blue powder:
- ant-zn-1p: 1 x 1 g

Recent articles using InvivoGen Zeocin™

• 2017 - Nature., 543(7646):568-572.
  DND1 maintains germline stem cells via recruitment of the CCR4–NOT complex to target mRNAs.
  Yamaji M. et al.
• 2017 - Cell., 168(3):503-516.
  Pathogen-Mediated Inhibition of Anorexia Promotes Host Survival and Transmission.
  Rao S. et al.
• 2017 - Nat Commun., 8:14391.
  IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP.
  Jønsson KL. et al.
• 2017 - Sci Rep., 7:44328.
  Plectin is a novel regulator for apical extrusion of RasV12-transformed cells
  Kadeer A. et al.
• 2017 - PNAS, [Epub ahead of print]
  Rational design of adjuvants targeting the C-type lectin Mincle.
  Decout A. et al.