货号: | 47-KIT 036 |
样本: | 尿液、血浆、血清、组织提取物或细胞上清 |
适应物种: | 人 |
应用: | ELISA |
检测方法: | 夹心法 |
检测限: | 10-1000pg/ml |
供应商: | 华泰昕 |
数量: | 期货 |
规格: | 96wells |
描述:
NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.
特点:
· Fast results from up to 40 samples in duplicate in just 4 hours per 96 well plate
· Highly sensitive measurement, detecting as low as 4 pg/ml of NGAL
· Ready-to-use pre-coated plate and individual calibrators save time and reduce errors
· Fully quantitative results that surpass semi-quantitative Western blot analysis
试剂盒成分:
1: 12 strips of 8 Microwells each coated with anti-NGAL (96 wells) + frame
2: Sample Diluent
3: NGAL Calibrators 1-8; 0, 10, 25, 50, 100, 250, 500, 1000 pg/mL
4: 25X Wash Solution Concentrate
5: Biotinylated NGAL Antibody
6: HRP-Streptavidin Conjugate
7: TMB Substrate
8: Stop Solution
- Product name
- NGAL ELISA Kit (human)
- Description
-
An ELISA for the in vitro determination of human NGAL in urine, serum, plasma, tissue extracts or culture media.
- Assay range
-
10-1000 pg/mL
- Assay time
- 4 hours
- Limit of detection
-
4 pg/mL
- Sample dilution
- 1/500 (Urine from healthy subjects)
- Storage
- 4°C
- Target
-
Human NGAL (Neutrophil Gelatinase-Associated Lipocalin)
- Background
-
NGAL is a new early biomarker for diagnosing acute kidney injury (AKI)
Under normal conditions, NGAL levels are low in urine and plasma, but they rise sharply from basal levels in response to kidney injury to reach diagnostic levels within a very short time - as much as 24 hours or more before any significant rise in serum creatinine.
- Regulatory status
- Not available in the USA for in vitro diagnostic use. For in vitro diagnostic use in the European Union. For research only on all other markets.
- Other information
- Simple ELISA format - easy to perform Ready-to-use calibrators and working solutions Pre-coated ELISA strips All incubations performed at room temperature Easy storage All components are stored at 4°C Automation possible on open ELISA workstations