货号: | CA001 |
供应商: | 南京赛博生物科技有限公司 |
保质期: | 1年 |
规格: | 20T |
- Kit contents:
Cat.No | CA001-1 | CA001-2 | CA001-3 |
Size | 20T | 50T | 100T |
Annexin V-FITC | 100ul | 250ul | 500ul |
Propidium Iodide | 200ul | 500ul | 1ml |
1x Binding Buffer | 20ml | 40ml | 80ml |
- Binding Buffer
- Propidium Iodide
- Annexin V-FITC
Molecular Weight:35.8 KDa
Storage: store in solution(50mM TRIS, 100mM NaCl, 1%BSA, 0.02%NaN3,pH7.4).
Purity: >98%
Biologic activity: Annexin V can be combined with phosphatidyl serine and shows activity of anti phospholipase.
- Apoptosis detection application(FCM):
- Centrifugate and collect the Suspension cells,discard the medium.
3. Add 5ul Annexin V-FITC to the Suspension cells. Gently mix the solution and incubate it for 10~20mins at 2~8℃away from light;
4. Then add 10ul Propidium iodide. Gently mix the solution and incubate it for 5mins at 2~8℃away from light;
- Then analyse using FACS or Fluorescence microscope within 1 hour.
Figure of Jurka cell cisplatin induced detected using Annexin V-FITC/PI