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Monoclonal anti-Human, IRF-5 antibo
Phalloidin:FITC
S100B antibody
[VIP第1年] 指数:2
通过广州市黄埔区市场和质量监督管理局认证 
TETRAMETHYLRHODAMINE| 货号： | S7350 |
| 宿主： | ot |
| 适应物种： | , |
| 应用范围： | , |
| 是否单克隆： | 否 |
| 抗原来源： | ot |
| 数量： | 大量 |
| 供应商： | MILLIPORE |
The OxyICC™ kit provides reagents for immunodetection of protein carbonyls by fluorescent immunocytochemistry. The assay procedure is simple and the results obtained are highly sensitive and quantitative.
OxyICC Detection of Oxidative Stress. HeLa cells were cultured with or without hydrogen peroxide (H2O2; 400µM) for 30 minutes. The cells were then analyzed for oxidative stress following the OxyICC™ protocol. Control reactions without DNPH treatment were also run to assess background. The test reactions were as follows: (A) -H2O2/ -DNPH (B) +H2O2/-DNPH (C) -H2O2/+DNPH and (D) +H2O2/+DNPH. Cell nuclei are blue due to DAPI staining whereas DNP signal is red. Virtually no staining was observed in the control reactions A and B which lack DNPH treatment. Minimal signal was observed in reaction C which is indicative of basal levels of oxidation that is present under normal cellular conditions. Treatment of cells with hydrogen peroxide in reaction D induces a strong fluorescent signal indicating extensive oxidative stress.
Quantitation of OxyICC™ Analysis. Three independent experiments were performed using HeLa cells and hydrogen peroxide (H2O2) treatment. The test reactions were: (A) -H2O2/-DNPH (B) +H2O2/-DNPH (C) -H2O2/+DNPH and (D) +H2O2/+DNPH. For each test reaction the Cy3 fluorescence pixel intensity was quantified for ten randomly chosen cells using Leica’s LAS AF software. The signals obtained were averaged and then normalized to background to give a signal intensity measurement for each experiment. The data for the three experiments was then averaged and is depicted above with error bars. The quantitative measurement shows that the +H2O2/+DNPH reaction (D) had over a four fold signal intensity increase versus basal levels found in the -H2O2/+DNPH sample (C).
