货号: | ant-zn-1 |
CAS号: | 11006-33-0 |
供应商: | 上海伟进生物科技有限公司 |
数量: | 现货 |
英文名: | Zeocin |
保质期: | 1 years |
保存条件: | 4°C或-20°C保存1年,避免反复冻融 |
规格: | 1g (10 x 1 ml)/1 g (powder) |
Zeocin化学特性
CAS号:11006-33-0
分子量:1525
推荐使用浓度
大肠杆菌
Zeocin抗性的转化子可以在低盐LB琼脂糖培养基(yeast extract 5 g/l, Tryptone 10 g/l, NaCl 5 g/l, Agar 15 g/l,pH 7.5),25 ug/ml Zeocin的条件下筛选。invivogen公司的FastMedia™ Zeo培养基可助您得到最佳筛选效果。
哺乳动物细胞
细胞系 | 种属 | 组织 | 培养基 | Zeocin(ug/ml) |
B16 | mouse | melanoma | RPMI | 20-250 |
CHO | hamster | ovary | DMEM | 100-500 |
COS | monkey | kidney | DMEM | 100-400 |
293 | human | kidney | DMEM | 100-400 |
Hela | human | uterus | DMEM | 50-100 |
J558L | mouse | melanoma | RPMI | 400 |
MCF-7 | human | Breast adenocarcinoma | DMEM | 100-400 |
MEFs | mouse | embryonic fibroblasts | DMEM | 200-400 |
THP-1 | human | Leukemic monocytes | RPMI | 200 |
质量控制:
纯度>90%(HPLC)
活性经微生物学实验鉴定
保存
4°C或-20°C保存1年,避免反复冻融。
文献引用
•2011 - PNAS, 108: 16663 - 16668
CD147 subunit of lactate/H+ symporters MCT1 and hypoxia-inducible MCT4 is critical for energetics and growth of glycolytic tumors.
Le Floch R, Chiche J, et al.
•2011 - Infect. Immun., 79: 1418 - 1427
NOD-like receptor activation by outer membrane vesicles from Vibrio cholerae non-O1 non-O139 strains is modulated by the quorum-sensing regulator HapR.
Bielig H, Rompikuntal PK, et al.
•2012 - PNAS, 109: 78 - 83
Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.
Sandstrom AG, Wikmark Y, et al.
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